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舟山近海棘头梅童鱼群体遗传多样性微卫星DNA分析
引用本文:林能锋,苏永全,丁少雄,王军.舟山近海棘头梅童鱼群体遗传多样性微卫星DNA分析[J].台湾海峡,2011,30(2):229-233.
作者姓名:林能锋  苏永全  丁少雄  王军
作者单位:1. 厦门大学海洋与环境学院,福建,厦门,361005;福建省农业科学院生物技术研究所,福建,福州,350003
2. 厦门大学海洋与环境学院,福建,厦门,361005
基金项目:国家863计划资助项目,福建省农业科学院青年科技人才创新基金
摘    要:应用6对可在棘头梅童鱼(Collichthy lucidels)中扩增的大黄鱼(Pseudosciaena Crocea)微卫星引物对浙江舟山近海的棘头梅童鱼群体进行PCR扩增,变性聚丙烯酰胺凝胶电泳分析扩增产物.6对引物在群体中共扩增出46个等位基因,平均每个位点得到5.308 0个有效等位基因,各位点的PIC值为0.412 6~0.893 5(平均值0.670 8),PC1C4和PC8F5为中度多态性位点,PC4H12、PC5E11、PC10F10及PC10G6为高度多态性位点,这些位点可以做为棘头梅童鱼群体遗传学研究的有效的分子遗传标记.6个位点的连锁分析显示,各位点间不存在明显的连锁关系.各位点在群体中的观测杂合度(Ho)为0.454 5~0.916 7(平均值0.659 1),期望杂合度(He)为0.468 0~0.901 9(平均值0.699 7),与其他海水鱼类比较,浙江近海的棘头梅童鱼群体遗传多样性偏低.对各位点进行Hardy-Weinberg平衡检测表明,PC4H12、PC10F10及PC10G6位点的等位基因频率偏离了平衡,综合现有的资源调查资料,遗传多样性的降低与近年来棘头梅童鱼资源的下降有关.

关 键 词:海洋生物学  棘头梅童鱼  大黄鱼  微卫星  遗传多样性  舟山近海

Genetic diversity assessed using microsatellite DNA of spiny head croaker (Collichthys lucidus) from the Zhoushan offshore waters
LIN Neng-feng,SU Yong-quan,DING Shao-xiong,WANG Jun.Genetic diversity assessed using microsatellite DNA of spiny head croaker (Collichthys lucidus) from the Zhoushan offshore waters[J].Journal of Oceanography In Taiwan Strait,2011,30(2):229-233.
Authors:LIN Neng-feng  SU Yong-quan  DING Shao-xiong  WANG Jun
Institution:LIN Neng-feng1,2,SU Yong-quan1,DING Shao-xiong1,WANG Jun1(1.College of Oceanography and Environmental Science,Xiamen University,Xiamen 361005,China,2.Institute of Biotechnology,Fujian Academy of Agriculture Sciences,Fuzhou 350003,China)
Abstract:Spiny head croaker(Collichthy lucidus) is a demersal and diminutive fish species,and was an abundant resource in the East China Sea until about 20 years ago.With the development of marine fisheries,much of the breeding stock of spiny head croaker and juvenile populations were overfished employing the traditional two-stick stow net method.To propose reasonable protection of the C.lucidus resource,it is important to understand the background of its population genetics.In this study,samples of spiny head croak...
Keywords:marine biology  Collichthy lucidus  Pseudosciaena crocea  microsatellite  genetic diversity  Zhoushan offshore waters  
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