| 毛蚶受精和早期卵裂过程核行为的荧光显微镜观察 |
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| 引用本文： | 董迎辉,张晓,姚韩韩,林志华.毛蚶受精和早期卵裂过程核行为的荧光显微镜观察[J].台湾海峡,2011,30(2):223-228. |
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| 作者姓名： | 董迎辉 张晓 姚韩韩 林志华 |
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| 作者单位： | 1. 浙江万里学院生物与环境学院,浙江,宁波,315100
2. 上海海洋大学水产与生命学院,上海,201306 |
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| 基金项目： | 国家科技支撑计划资助项目，宁波市重大科研攻关项目 |
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| 摘 要： | 利用Hoechst33258染色、荧光显微镜观察方法,对毛蚶受精和早期卵裂过程中核行为的细胞学变化进行了详细观察.结果表明,毛蚶成熟未受精卵呈卵圆形,卵径为52.909±2.092μm,核相处于第一次成熟分裂中期.在水温为26±1℃条件下进行受精,在受精后15~18、20~25 min,受精卵先后排出第一、第二极体,完成第一次和第二次成熟分裂;受精后30 min左右,雌、雄性原核形成;受精后35 min,雌、雄性原核各自形成染色体组,在卵子中央发生联合,染色体共同排列在纺锤体的赤道板上,形成第一次有丝分裂的中期分裂相;受精后40 min左右,受精卵进行第一次卵裂,形成2个大小不等的卵裂球;受精后55~60 min,受精卵完成第二次卵裂,形成1大3小4个卵裂球.另外,在研究中还发现了极少量的多精受精、多极分离等异常细胞学现象,对其成因及机制进行了分析和探讨.
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| 关 键 词： | 海洋生物学 毛蚶 受精 早期卵裂 核行为 荧光显微镜 |
Cytological observation of nuclear behavior in fertilization and early cleavage in Scapharca subcrenata |
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| DONG Ying-hui,ZHANG Xiao,YAO Han-han,LIN Zhi-hua.Cytological observation of nuclear behavior in fertilization and early cleavage in Scapharca subcrenata[J].Journal of Oceanography In Taiwan Strait,2011,30(2):223-228. |
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| Authors: | DONG Ying-hui ZHANG Xiao YAO Han-han LIN Zhi-hua |
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| Institution: | DONG Ying-hui1,ZHANG Xiao1,YAO Han-han2,LIN Zhi-hua1(1.College of Biological & Environmental Sciences,Zhejiang Wanli University,Ningbo 315100,China,2.College of Fishery and Life Sciences,Shanghai Ocean University,Shanghai 201306,China) |
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| Abstract: | Scapharca subcrenata is distributed from the north to the south coast of China and has become one of the most popular commercially important bivalves in aquaculture as it grows rapidly,has high-quality meat,and is easy to farm.Although there have been numerous studies on the reproductive biology of S.subcrenata,there has been scarce research on fertilization and early development.This paper consecutively investigates cytological changes in nuclear behavior in the process of fertilization and early embryonic... |
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| Keywords: | marine biology Scapharca subcrenata fertilization early cleavage nuclear behavior fluorescent microscope |
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