| 氨氮胁迫对拟穴青蟹组织器官中SOD及GPX活性的影响 |
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| 引用本文： | 曾媛媛,蒋云霞,艾春香.氨氮胁迫对拟穴青蟹组织器官中SOD及GPX活性的影响[J].台湾海峡,2011,30(2):210-215. |
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| 作者姓名： | 曾媛媛 蒋云霞 艾春香 |
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| 作者单位： | 1. 厦门大学海洋与环境学院,福建,厦门,361005
2. 南方医科大学公共卫生与热带医学院,广东,广州,510515 |
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| 基金项目： | 国家863计划资助项目，国家科技部公益性行业科研专项资助项目，福建省科技计划重点资助项目 |
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| 摘 要： | 采用实验生态学的方法,探讨海水中分别添加氨氮（以氯化铵作为氨氮源）0（对照组,C0组）、10（C10组）、20（C20组）、30（C30组）、40 mg/dm3（C40组）,胁迫24、48、72、96 h对拟穴青蟹（Scylla paramamosain）各组织器官中SOD、GPX活性的影响.结果表明,拟穴青蟹鳃中SOD活性,除C40组外,其余各组在胁迫24、48 h随氨氮含量升高而升高,胁迫72、96 h则随氨氮含量升高而下降（p〈0.05）,C40组的则随胁迫时间延长而一直呈下降趋势;各组拟穴青蟹鳃、肝胰腺和肌肉的SOD活性变化趋势相同,鳃和肌肉SOD活性最高均出现在胁迫48 h,肝胰腺SOD活性最高则在胁迫72 h.各组拟穴青蟹鳃GPX活性在胁迫24、48和72 h随氨氮含量升高而下降,胁迫96 h随氨氮含量升高而增大;各组拟穴青蟹肝胰腺和肌肉GPX活性变化与肝胰腺SOD活性变化趋势相似,胁迫48 h,C40组拟穴青蟹肝胰腺GPX活性最高.氨氮胁迫显著影响拟穴青蟹生理效应.
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| 关 键 词： | 海洋生物学 拟穴青蟹 氨氮胁迫 超氧化物歧化酶 谷胱甘肽过氧化物酶 |
Effects of ammonia-N stress on the activities of superoxide dismutase and glutathione peroxidase in different tissues and organs of Scylla paramamosain |
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| ZENG Yuan-yuan,JIANG Yun-xia,AI Chun-xiang.Effects of ammonia-N stress on the activities of superoxide dismutase and glutathione peroxidase in different tissues and organs of Scylla paramamosain[J].Journal of Oceanography In Taiwan Strait,2011,30(2):210-215. |
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| Authors: | ZENG Yuan-yuan JIANG Yun-xia AI Chun-xiang |
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| Institution: | ZENG Yuan-yuan1,JIANG Yun-xia2,AI Chun-xiang1(1.College of Oceanography and Environmental Science,Xiamen University,Xiamen 361005,China,2.School of Public Health and Tropical Medicine,Southern Medical University,Guangzhou 510515,China) |
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| Abstract: | In an intensive culture system,accumulation of ammonia-N,the principal end product of nitrogenous metabolism,has deleterious effects on the metabolism,growth,health and reproduction of aquatic animals.The aim of the present work was to study the physiological response of Scylla paramamosain(Estampador,1949) to ambient ammonia stress.An experimental ecology method was employed to investigate the effects of ammonia-N 0(control group),10,20,30,40 mg/dm3 stress on the activities of superoxide dismutase(SOD) and... |
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| Keywords: | marine biology Scylla paramamosain ammonia-N stress superoxide dismutase glutathione peroxidase |
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