| 杂色鲍血蓝蛋白HtH1基因第16内含子SNP密度的分析 |
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| 引用本文： | 梁翠,陈军,游伟伟,王德祥,柯才焕.杂色鲍血蓝蛋白HtH1基因第16内含子SNP密度的分析[J].台湾海峡,2011,30(1):21-26. |
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| 作者姓名： | 梁翠 陈军 游伟伟 王德祥 柯才焕 |
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| 作者单位： | 厦门大学海洋与环境学院,福建,厦门,361005 |
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| 基金项目： | 国家自然科学基金资助项目 |
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| 摘 要： | 单核苷酸多态性（SNPs）是继RFLP和SSR多态性标记之后的新一代遗传标记系统.本文采用EPIC的方法,分析了45个杂色鲍样品的血蓝蛋白HtH1基因第16内含子（H1 intron 16）的SNP数据.这45个个体分别来自日本、台湾和汕头3个不同海域的野生或养殖群体.日本群体的血蓝蛋白HtH1基因第16内含子呈现出长度多态性,共有3种不同长度,分别是1 900、780、440 bp,测序结果表明3个等位基因可以互相比对;而台湾和汕头杂色鲍养殖群体的均为1 900 bp.初步研究结果表明日本杂色鲍个体大约96 bp长的片段含有1个自身杂合SNP位点;台湾杂色鲍个体约133 bp含有1个自身杂合SNP位点;汕头杂色鲍个体约153 bp含有1个自身杂合SNP位点.13个台湾杂色鲍个体包含的SNP密度为约31 bp含有1个SNP;23个汕头杂色鲍个体包含的SNP密度为约21 bp含有1个SNP.这些数据表明现有的杂色鲍养殖群体包含了丰富的SNP位点,利用SNP分子标记进行高密度遗传图谱的构建是可行的.
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| 关 键 词： | 海洋生物学 SNP EPIC 血蓝蛋白基因HtH1 杂色鲍 |
SNP density of hemocyanin HtH1 intron 16 in Haliotis diversicolor |
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| LIANG Cui,CHEN Jun,YOU Wei-wei,WANG De-xiang,KE Cai-huan.SNP density of hemocyanin HtH1 intron 16 in Haliotis diversicolor[J].Journal of Oceanography In Taiwan Strait,2011,30(1):21-26. |
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| Authors: | LIANG Cui CHEN Jun YOU Wei-wei WANG De-xiang KE Cai-huan |
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| Institution: | (College of Oceanography and Environmental Science,Xiamen University,Xiamen 361005,China) |
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| Abstract: | Single nucleotide polymorphisms（SNP） are a new generation of genetic marker systems.EPIC was used to analyze the SNP of the hemocyanin HtH1 gene 16 intron（H1 intron 16） from 45 samples of Haliotis diversicolor.These 45 individuals came from three different wild or breeding populations in Japan,Taiwan and the Shantou Sea.There were three alleles in H1 intron 16 of the Japanese abalone（Haliotis diversicolor）,which showed length polymorphism.The lengths of the three alleles were 1 900 bp,780 bp and 440 bp.However,the H1 intron 16 of the breeding populations of Taiwan and Shantou abalone were only 1 900 bp.Sequence analysis indicated that there was a self-heterozygous SNP locus of approximately 96 bp in the Japanese abalone,but that the Taiwan and Shantou abalone contained loci of 133 bp and 153 bp,respectively.The SNP density of 13 Taiwan abalone was 31 bp,while the SNP density of 23 Shantou abalone was 21 bp.These data indicated that the existing cultured population of abalone contained rich SNP loci,and it was feasible to construct a high density genetic map using SNP markers. |
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| Keywords: | SNP EPIC |
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