| 二种体色拟穴青蟹群体ISSR分析 |
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| 引用本文： | 蔡小辉,宋忠魁,彭银辉,聂振平,赵鹏,苏琼.二种体色拟穴青蟹群体ISSR分析[J].台湾海峡,2011,30(3):363-368. |
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| 作者姓名： | 蔡小辉 宋忠魁 彭银辉 聂振平 赵鹏 苏琼 |
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| 作者单位： | 广西海洋研究所;广西海洋生物技术重点实验室,广西北海536000 |
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| 基金项目： | 广西自然科学基金资助项目(桂科自0991189); 广西教育厅科研课题经费资助项目(200809MS095); 广西海洋生物技术重点实验室开放基金资助项目(GKLMBT-0801) |
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| 摘 要： | 应用ISSR分子标记技术分析二种体色拟穴青蟹群体的遗传多样性．10条ISSR引物共扩增出81条带，其中73条表现出多态性，占总条带数的90．12％．暗红色和深绿色拟穴青蟹群体的多态性位点比率分别为81．48％和77．78％，暗红色群体的Nei’S基因多样性指数（He=0．3189）和Shannon’S信息指数（，=0．4690）略高于深绿色群体（He：0．3054，，=0．4491）．群体间的Nei’S遗传相似性（0．9484）和遗传距离（0．0529）表明，群体间遗传分化属于种内遗传分化．POPGENE和AMOVA分析表明，总的遗传变异主要来源于群体内个体间，群体间发生中等程度遗传分化，群体间存在强大的基因流．UPGMA聚类图可视化群体间和群体内的遗传变异，显示60个个体并没有按体色聚类．
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| 关 键 词： | 海洋生物学 拟穴青蟹 体色 1SSR 遗传多样性 |
ISSR analysis of two Scylla paramamosain populations with different body colors |
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| CAI Xiao-hui,SONG Zhong-kui,PENG Yin-hui,NIE Zhen-ping,ZHAO Peng,SU Qiong.ISSR analysis of two Scylla paramamosain populations with different body colors[J].Journal of Oceanography In Taiwan Strait,2011,30(3):363-368. |
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| Authors: | CAI Xiao-hui SONG Zhong-kui PENG Yin-hui NIE Zhen-ping ZHAO Peng SU Qiong |
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| Institution: | CAI Xiao-hui,SONG Zhong-kui,PENG Yin-hui,NIE Zhen-ping,ZHAO Peng,SU Qiong (Guangxi Key Laboratory for Marine Biotechnology,Guangxi Institute of Oceanology,Beihai 536000,China) |
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| Abstract: | The genetic diversity and population genetic structure of two Scylla paramamosain(Estampador,1949) populations with different body colors sampled from the Thanh Hóa Province in Vietnam were surveyed using the molecular marker technique of inter-simple sequence repeats(ISSR).A total of 81 loci of 60 individuals were amplified by PCR with 10 optimal ISSR primers,among which 73 loci(90.12%) were polymorphic.The percentages of polymorphic loci in the dark red and bottle green populations were 81.48% and 77.78%,respectively.Nei’s gene diversity(He=0.318 9) and Shannon’s information index(I=0.469 0) of the dark red population were slightly higher than those of the bottle green population(He=0.305 4,I=0.449 1).The Nei’s gene diversity and Shannon’s information index of S.paramamosain were 0.312 2 and 0.459 1 at the population level,and 0.332 4 and 0.493 1 at species level,respectively,showing a relatively high genetic diversity of S.paramamosain.Analysis of the molecular variance(AMOVA) demonstrated that the inter-population component accounted for 8.73% of the total variation,while the intra-population component accounted for 91.27%,which demonstrated that the total genetic variation mainly originated from within the populations.The genetic differentiation coefficient(Gst),gene flow(Nm) and the average Nei’s genetic distance were 0.060 9,7.704 6,and 0.052 9,respectively,between the two populations.The study indicated a medium degree of genetic divergence and a thriving gene flow between the two populations;however,such divergence did not surpass the species level.The genetic variation between or within populations was visualized using a UPGMA dendrogram,which revealed that similar body color individuals did not cluster together.Taken together,the body color of S.paramamosain had no relation to the genetic structure of the populations,and the difference is probably related to a difference in habitats |
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| Keywords: | marine biology Scylla paramamosain body color inter-simple sequence repeats(ISSR) genetic diversity |
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