| 东南太平洋热液区硫化物样品富集培养物中Fe3＋还原细菌的多样性分析 |
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| 引用本文： | 秦丹,张晓波,姜丽晶,盖英宝,曾湘.东南太平洋热液区硫化物样品富集培养物中Fe3＋还原细菌的多样性分析[J].台湾海峡,2011,30(3):379-385. |
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| 作者姓名： | 秦丹 张晓波 姜丽晶 盖英宝 曾湘 |
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| 作者单位： | 国家海洋局第三海洋研究所,福建厦门,361005 |
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| 基金项目： | 福建省自然科学基金资助项目(2010J05097); 国家海洋局第三海洋研究所基本科研业务费专项资金资助项目(海三科2010008) |
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| 摘 要： | 采集了东南太平洋热液区DYl15-20V一$35一TVGl7站位的沉积物硫化物样品，其含有76．717％（m／m）强还原性的磁性良好的黄铁矿．以乳酸钠为电子供体，不溶性的氢氧化铁为电子受体，对该样品进行Fe¨还原细菌的定向富集培养．通过构建该茵群的16SrDNA基因文库，随机挑选82个阳性克隆子进行群落结构多样性分析．研究结果表明：82个克隆代表的6种基因型分别属于4个主要类群，其中ε一变形杆菌占有绝对优势，达到55．2％．此外其还包括厚壁菌门、β一变形杆菌和仅．变形杆菌，克隆子比例依次为27．6％、10．3％、6．9％．在富集产物中还原氢氧化铁的功能菌群主要是具有硫还原作用的ε一变形菌中的硫还原菌．
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| 关 键 词： | 海洋生物学 热液硫化物 x射线衍射（XRD） Fe3＋还原菌 群落结构 多样性 东南太平洋 |
Diversity analysis of Fe3+ -reducing bacteria in enriched cultures from hydrothermal sulfide sample of southeast Pacific Ocean |
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| QIN Dan,ZHANG Xiao-bo,JIANG Li-jing,GAI Ying-bao,ZENG Xiang.Diversity analysis of Fe3+ -reducing bacteria in enriched cultures from hydrothermal sulfide sample of southeast Pacific Ocean[J].Journal of Oceanography In Taiwan Strait,2011,30(3):379-385. |
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| Authors: | QIN Dan ZHANG Xiao-bo JIANG Li-jing GAI Ying-bao ZENG Xiang |
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| Institution: | QIN Dan,ZHANG Xiao-bo,JIANG Li-jing,GAI Ying-bao,ZENG Xiang(Third Institute of Oceanography,SOA,Xiamen 361005,China) |
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| Abstract: | The ocean contains rich resources and in deep sea sediments there exists iron compounds at different stages of oxidation and reduction.Iron is one of the most widely distributed elements on the earth and its cycle chiefly includes two processes of oxidation and reduction.As a primitive form of respiration,Fe3+-reduction and Fe3+-reducing bacteria play an important role in the iron cycle where in sediments with an anaerobic environment,where iron chiefly exists in a reduced form.In the current study,a hydrot... |
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| Keywords: | marine biology hydrothermal sulfide X-ray diffraction(XRD) Fe3 -reducing bacteria structure of the biotic community diversity southeast Pacific Ocean |
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