| 九龙江口红树植物分子分类的初步研究 |
| |
| 引用本文： | 周涵韬,林鹏,郑文竹,孙晟,郑志强,林凡,陈鹭真.九龙江口红树植物分子分类的初步研究[J].台湾海峡,2001,20(1):66-71,T005. |
| |
| 作者姓名： | 周涵韬 林鹏 郑文竹 孙晟 郑志强 林凡 陈鹭真 |
| |
| 作者单位： | 厦门大学生物系, |
| |
| 基金项目： | 国家教育部高等学校博士点基金资助项目(1999038410) |
| |
| 摘 要： | 本研究工作以福建九龙江口龙海红树林自然保护区浮宫种苗园内7种红树植物为材料，采用改进的CTAB法获得了纯度较高(A260/A280在1.6～2.0之间)，得率高(DNA平均得率约为120×10－6m/m，FreshWeight)，片段完整(片段大小约为23kb左右)的基因组DNA，并运用RAPD技术对这7种红树植物进行了遗传多样性分析．从30个10-mer随机引物中筛选出9个有效引物，并利用这9个有效引物共扩增出352条DNA带.利用UPGMA法对红树植物的7个种间的亲缘关系进行聚类分析，得出7个种的DNA分子分类系统图,并与传统的分类进行比较,发现结果相符，从而确定了RAPD技术用于红树植物分子分类研究的可行性，并为从分子水平研究红树植物遗传多样性,保护、开发和利用红树林资源提供科学依据.
|
| 关 键 词： | 红树植物 RAPD 分子分类 引种 遗传多样性 |
| 文章编号： | 1000-8160（2001）01-0066-06 |
Molecular classification
of seven species of mangroves in Jiulongjiang Estuary in Fujian |
| |
| ZHOU Han-tao,LIN Peng,ZHENG Wen-zhu,SUN Sheng,ZHENG Zhi-qiang,Lin Fan,ChEN Lu-zhen.Molecular classification
of seven species of mangroves in Jiulongjiang Estuary in Fujian[J].Journal of Oceanography In Taiwan Strait,2001,20(1):66-71,T005. |
| |
| Authors: | ZHOU Han-tao LIN Peng ZHENG Wen-zhu SUN Sheng ZHENG Zhi-qiang Lin Fan ChEN Lu-zhen |
| |
| Abstract: | DNAs from 7 species of mangroves were extracted by the CTAB method. The A 260 / A 280 value of DNA solutions ranged from 1.6 to 2.0. RAPD analysises on genetic diversity of mangroves were conducted. 9 effective primers were screened from 30 arbitrary primers, and 352 DNA bands were amplified. Based on UPGMA cluster analysis on DNA bands amplified by 9 primers, a DNA molecular dendrogram was established. Compared to the conventional classification, the molecular classification of mangroves was accurate.WT5F1] |
| |
| Keywords: | mangroves RAPD molecular classification |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
