| 文蛤多肽的体外抗癌活性研究 |
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| 引用本文： | 范成成,张剑,康劲翮,邱乒乒,韩鹏,李文岗,陈清西.文蛤多肽的体外抗癌活性研究[J].台湾海峡,2009,28(4):472-476. |
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| 作者姓名： | 范成成 张剑 康劲翮 邱乒乒 韩鹏 李文岗 陈清西 |
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| 作者单位： | 1. 厦门大学生命科学学院、细胞生物学与肿瘤细胞工程教育部重点实验室,福建厦门,361005
2. 福建医科大学附属厦门第一医院,福建厦门,361004 |
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| 基金项目： | 厦门市科技计划资助项目，厦门市卫生局资助项目，厦门大学科技创新工程基金资助项目 |
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| 摘 要： | 采用硫酸铵沉淀、有机溶剂分离、Sephadex G-25分子筛层析等实验方法，从文蛤肉中提取了一种低分子量的多肽，命名为Mer2．本文以溴化二苯偶氮盐（MTT）法检测Mer2对体外培养的癌细胞的抑制作用．结果表明Mer2对体外培养的人肝癌细胞株（HepG2）、宫颈癌细胞株（Hela）、胆管癌细胞株（QBC939）、肺癌细胞株（SPC—A-1和LTEP—a-2）的生长均有很强的抑制作用，且抑制效果随着Mer2含量的增高和处理时间的延长而增强，证明Mer2具有广谱的抗癌活性．其中Mer2对人肝癌HepG2细胞株抑制作用最为显著，光学显微镜观察经Mer2细胞培养液处理后的细胞形态发生明显的改变，流式细胞仪实验的结果表明处理后的细胞周期发生明显的改变，并在G0／G1期前出现凋亡峰．
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| 关 键 词： | 海洋生物学 细胞 文蛤多肽 抗癌 MTT比色法 凋亡 |
Antitumor activity of peptide from Meretrix meretrix in vitro |
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| FAN Cheng-cheng,ZHANG Jian,KANG Jing-he,QIU Ping-ping,HAN Peng,LIN Wen-gang,CHEN Qing-xi.Antitumor activity of peptide from Meretrix meretrix in vitro[J].Journal of Oceanography In Taiwan Strait,2009,28(4):472-476. |
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| Authors: | FAN Cheng-cheng ZHANG Jian KANG Jing-he QIU Ping-ping HAN Peng LIN Wen-gang CHEN Qing-xi |
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| Institution: | FAN Cheng-cheng , ZHANG Jian , KANG Jing-he, QIU Ping-ping, HAN Peng, LIN Wen-gang, CHEN Qing-xi ( 1. Key lab of ministry of Education for Cell Biology and Tumor Cell Engineering,Xiamen University,Xiamen 361005 ,China; 2. Xiamen First Hospital attached to Fujian Medical University, Xiamen 361004, China) |
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| Abstract: | An anti-cancer peptide was purified from Mercenaria(Meretrix meretrix)by methods of fragmentation,organic precipitation and column chromatogram (Sephadex G-25).Finally,the anticancer peptide was obtained and named as Mer2.The antitumor activity and stability of Mer2 were preliminarily determined by MTT method in vitro.It shows that Mer2 inhibited significantly the growth of human cancer cells including HepG_2,HeLa,QBC939,SPC-A-1 and LTEP-a-2 in a dose-dependent manner in vitro,and had a more remarkable inhibitory effect on liver cancer cell strain HepG_2.The cell morphology of HepG_2 treated by the peptide Mer2 was changed distinctly under the LM and the proliferation of HepG_2 cells through induced-apoptosis was changed clearly showed by flow cytometry. |
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| Keywords: | marine biology cell Meretrix meretrix peptide antitumor MTT colorimetry apoptosis |
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