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| 福建太平洋牡蛎种群微卫星DNA分析 | |
| 引用本文： | 陈曦,黄勤,江锦祥,马平.福建太平洋牡蛎种群微卫星DNA分析[J].台湾海峡,2007,26(4):548-554. |
| 作者姓名： | 陈曦 黄勤 江锦祥 马平 |
| 作者单位： | 1. 福建省农业科学院,福建,福州,350003 2. 国家海洋局第三海洋研究所,福建,厦门,361005 3. 福建省海洋与渔业局水产技术推广站,福建,福州,350003 |
| 摘 要： | 本文采用Ucdcg153、157、202微卫星引物对福建两个太平洋牡蛎(学名为长牡蛎Crassostrea gigas)养殖种群进行扩增分析和序列测定.与采自漳浦旧镇的近江牡蛎(C. ariakensis)比较,前者对以上三种引物全部呈阳性反应,后者只对Ucdcg157显阳性.与Genebank提供的相关序列比较,福建太平洋牡蛎与Genebank样品应属同源,其中漳浦霞美和厦门白礁的样品则可能代表同一种群的两个衍生品系.本项调查结果同时显示,近亲繁殖的育苗方式已经导致福建太平洋牡蛎种群裂化;任其发展不利于维护良种优势.本次采集的样品中未发现显示葡萄牙牡蛎、熊本牡蛎和美洲牡蛎微卫星特征的个体. |
| 关 键 词： | 太平洋牡蛎 近江牡蛎 微卫星DNA 种群裂化 |
| 文章编号： | 1000-8160（2007）04-0548-07 |
| 修稿时间： | 2006-12-31 |
Microsatellite sequence variation in the Pacific oyster Crassostrea gigas from south Fujian, China |
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| CHEN Xi,HUANG Qin,JIANG Jin-xiang,MA Ping.Microsatellite sequence variation in the Pacific oyster Crassostrea gigas from south Fujian, China[J].Journal of Oceanography In Taiwan Strait,2007,26(4):548-554. | |
| Authors: | CHEN Xi HUANG Qin JIANG Jin-xiang MA Ping |
| Institution: | 1. Fujian Academy of Agriculture Sciences, Fuzhou 350003, China; 2. Third Institute of Oceanography, SOA,Xiamen 361005, China; 3. Fujian Bureau of Oceanography and Fishery, Fuzhou 350003, China |
| Abstract: | In present studies,we used three microsatellite markers,i.e.,Ucdcg153,Ucdcg157 and Ucdcg202,to investigate sequence variations of two farmed populations of C.gigas from Fujian.All DNA samples from either population were well amplified in response to relevant primers.To the contrast,samples of a native cup oyster C.ariakenis failed PCR-electrophoretic inspections when exposed to primers for Ucdcg153 and Ucdcg202 loci.According to results of microsatellite sequence analyses,populations of C.gigas in Fujian should have the same origin as those which left the first sequence data for Ucdcg153,Ucdcg157 and Ucdcg202 loci in Genebank,while the present two Fujian populations,one collected from Xiamei,Zhangpu County,another from Baijiao,Xiamen,were possibly derived from one.Sequence discrimination between C.gigas from Xiamei and Baijiao revealed serious crisis of population fragmentation for C.gigas in Fujian.Inbred hatchery manipulation is considered to be responsible for this situation.No individuals of C.angulata,C.sikamea and C.virginica were detected by microsatellite characters during this work. |
| Keywords: | Pacific oyster Crassostrea giga C ariakensis microsatellite DNA genetic polymorphism |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |