| 高压技术在深海沉积物兼性嗜压菌的筛选和鉴定中的应用 |
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| 引用本文： | 游志勇,汤熙翔,肖湘.高压技术在深海沉积物兼性嗜压菌的筛选和鉴定中的应用[J].台湾海峡,2007,26(4):555-561. |
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| 作者姓名： | 游志勇 汤熙翔 肖湘 |
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| 作者单位： | 1. 厦门大学生命科学学院,福建,厦门,361005
2. 厦门大学生命科学学院,福建,厦门,361005;国家海洋局第三海洋研究所,福建,厦门,361005 |
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| 基金项目： | 国际海底区域研究开发项目 |
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| 摘 要： | 通过自行改进的高压培养罐及高压设备,对深海沉积物进行可培养微生物的筛选,获得6株具有较强耐受压力的细菌.16SrDNA的测序结果表明这些细菌分别属于6个不同的菌属.压力生长试验的结果表明这6株细菌在40MPa的条件下仍然具有较强的生长能力,属于兼性嗜压菌.对不同压力下生长的细菌做显微镜检,结果显示,除了一株芽孢杆菌在40MPa下的菌体形态发生了明显的变化外,其它5株细菌在压力条件下的菌体的分裂均没有受到明显的影响.
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| 关 键 词： | 高压技术 深海沉积物 兼性嗜压菌 细菌筛选 |
| 文章编号： | 1000-8160（2007）04-0555-07 |
| 修稿时间： | 2007-01-18 |
Application of hydrostatic technique in the isolation and characterization of piezophilic bacteria from deep-sea sediments |
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| YOU Zhi-yong,TANG Xi-xiang,XIAO Xiang.Application of hydrostatic technique in the isolation and characterization of piezophilic bacteria from deep-sea sediments[J].Journal of Oceanography In Taiwan Strait,2007,26(4):555-561. |
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| Authors: | YOU Zhi-yong TANG Xi-xiang XIAO Xiang |
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| Institution: | 1. School of Life Science, Xiamen University, Xiamen 361005, China; 2. Third Institute of Oceanography, SOA, Xiamen 361005, China |
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| Abstract: | Through hydrostatic technique,6 facultative piezophilic bacteria were isolated from the deep-sea sediments,which were from the east Pacific and south sea of China.BLAST results of 16S rDNA sequences indicated that the bacteria were defined to 6 different genus respectively.The tests of the optimal growth pressure showed that all of the bacteria were facultative piezophilic that could grow under 40MPa.It showed that under the high pressure the bacterial shapes showed no visual change compared with that in the atmosphere,except Bacillus sp.SS-1. |
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| Keywords: | hydrostatic technique deep-sea sediments facultative piezophilic bacteria isolation |
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