Total thiol redox status as a potent biomarker of PAH-mediated effects on mussels |
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| Institution: | 1. Programa de Pós-graduação em Ecologia de Ambientes Aquáticos e Continentais (PEA)/Nupélia, Universidade Estadual de Maringá (UEM), Av. Colombo, 5790, Zona 7, 87020-900 Maringá, PR, Brazil;2. Universidade Tecnológica Federal do Paraná (UTFPR), Campus Dois Vizinhos, Estrada para Boa Esperança, km 04, P.O. Box 157, 85660-000 Dois Vizinhos, PR, Brazil;3. Universidade Tecnológica Federal do Paraná (UTFPR), Campus Campo Mourão, Via Rosalina Maria dos Santos, 1233, P. O. Box 271, 87301-899 Campo Mourão, PR, Brazil;4. Departamento de Farmacologia, Universidade Federal do Paraná (UFPR), Rua Coronel Fco. H. dos Santos, s/n, P.O. Box 19031, 81531-990 Curitiba, PR, Brazil;1. Department of Biochemistry, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn (UWM), Micha?a Oczapowskiego 1a, Olsztyn, Poland;2. Department of Food Technology, Spanish National Research Council, Marine Research Institute, Vigo, Spain |
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| Abstract: | This laboratory study describes phenanthrene (Ph) and/or anthracene (An) ability to alter the total thiol redox status (TRS), via depletion of protein free thiols (PSH) and glutathione (GSH) levels, in gills of mussel Mytilus galloprovincialis, after a short-term (7 days) exposure period to each contaminant (at a final concentration of 0.1 mg L?1) or in a mixture (ration 1:1, at a final concentration of 0.2 mg L?1). A number of observable changes, like lysosomal membrane impairment (as detected via the neutral red retention time assay, primarily performed in haemocytes), enhancement of lipid peroxidation byproducts, increased nuclear abnormalities, inhibition of AChE and ALP activity, as well as a significant depletion of PSH and GSH were detected in gills of exposed mussels, in any case. Significant relationships occurred among TRS parameters with each change/stress indices measured in tissues of mussels, could reinforce the use of PSH as a potent biomarker. |
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