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Response of embryos of the american oyster, Crassostrea virginica, to heavy metal mixtures
Authors:John R MacInnes
Institution:National Marine Fisheries Service, Northeast Fisheries Center, Milford Laboratory, Milford, Connecticut 06460, USA
Abstract:The response of embryos of the American oyster, Crassostrea virginica, to various combinations of two or three metals (copper, mercury and zinc as the nitrates and chlorides) was studied in the laboratory at the optimal temperature and salinity regime for their development. The experimental design was a 3 × 3 × 3 factorial experiment using copper concentrations of 0,8 and 16 ppb; zinc concentrations of 0, 100 and 200 ppb and mercury concentrations of 0, 6 and 10 ppb. Highly significant toxic synergism was observed in the copper-zinc mixtures and three-metal mixtures of both salts, particularly at high concentrations. Although the chloride salt appeared to be slightly more toxic than the nitrate salt, the overall difference was not significant.
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