| 罗氏沼虾胚胎发育过程中同工酶的研究 |
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| 引用本文： | 卢建平,姜乃澄.罗氏沼虾胚胎发育过程中同工酶的研究[J].海洋学研究,2000,18(3):34-39. |
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| 作者姓名： | 卢建平 姜乃澄 |
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| 作者单位： | 浙江大学,生命科学学院,浙江,杭州,310012 |
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| 摘 要： | 采用聚丙烯酰胺凝胶电泳技术 ,对罗氏沼虾 Macrobrachium rosenbergii胚胎发育过程中5种同工酶系统的表达进行了分析。结果表明 ,在胚胎发育过程中 LDH、 MDH、 G6PD、Cytc.Oxidase和 EST均有明显的变化 ,同工酶酶谱随胚胎的发育渐趋复杂 ,酶活性也随之增加。膜内氵蚤状幼体期初为胚胎同工酶基因被激活的关键时期。EST酶谱变化过程同卵黄的消化过程有关。
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| 关 键 词： | 罗氏沼虾 卵 胚胎 同工酶 |
| 修稿时间： | 1999-07-20 |
The isozyme patterns in embryo development of the freshwater prawn Macrobrachium rosenbergh |
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| LU Jian-ping,JIANG Nai-cheng.The isozyme patterns in embryo development of the freshwater prawn Macrobrachium rosenbergh[J].Journal of Marine Sciences,2000,18(3):34-39. |
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| Authors: | LU Jian-ping JIANG Nai-cheng |
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| Abstract: | The patterns of five isozymic systems (lactate dehydrogenenase,malate dehydrogenenase,glucose\|6\|phospahate dehydrogenenase,cytochrome oxidase and esterase) were investigated with polyacrylamide gel electrophoresis during the embryonic development of the prawn \%Macrobrachium rosenbergii\% All these isozymic systems exhibited rich patterns of ontogenetic changes And the isozyme patterns were growing complicated with the developing embryos The early stage of inner protozoea was the key phase for the gene expression to be activated and the isozymes to be produced. And the possible relationships between variations of the esterase pattern and the digesting process of yolk were discussed |
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| Keywords: | Macrobrachium rosenbergii\% egg embryo isozyme |
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