| 海洋源拮抗细菌的分离、筛选及1株拮抗芽孢杆菌的鉴定 |
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| 引用本文： | 曾海燕,谢小保,黄小茉,李彩玲,欧阳友生,陈仪本.海洋源拮抗细菌的分离、筛选及1株拮抗芽孢杆菌的鉴定[J].海洋学研究,2011,29(4):89-94. |
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| 作者姓名： | 曾海燕 谢小保 黄小茉 李彩玲 欧阳友生 陈仪本 |
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| 作者单位： | 广东省微生物研究所微生物分析检测中心菌种保藏与应用重点实验室,广东广州,510070 |
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| 基金项目： | 广东省科技攻关资助项目 |
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| 摘 要： | 对来自于不同海域的红树树皮、海苔(海洋生物样)和海泥(海洋底质)样品进行细菌分离,并采用管碟法测试了分离出的海洋细菌对金黄色葡萄球菌Staphylococcus aureus、枯草芽孢杆菌Bacillus subtilis、大肠杆菌Escherichia coli、白色念珠菌Candida albicans、黑曲霉As...
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| 关 键 词： | 海洋拮抗细菌 抑菌活性 菌种鉴定 |
Isolation and screening of marine antagonistic bacteria and identification of a strain of antagonistic Bacillus sp. |
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| ZENG Hai-yan,XIE Xiao-bao,HUANG Xiao-mo,LI Cai-ling,OU-YANG You-sheng,CHEN Yi-ben.Isolation and screening of marine antagonistic bacteria and identification of a strain of antagonistic Bacillus sp.[J].Journal of Marine Sciences,2011,29(4):89-94. |
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| Authors: | ZENG Hai-yan XIE Xiao-bao HUANG Xiao-mo LI Cai-ling OU-YANG You-sheng CHEN Yi-ben |
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| Institution: | (Key Laboratory of Microbial Culture Collection and Application,Detection Center of Microbiology, Guangdong Institute of Microbiology,Guangzhou 510070,China) |
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| Abstract: | 72 strains of marine bacteria were isolated from marine samples collected at harbors and mangroves in Guangdong.These strains were screened for antimicrobial activities on Staphylococcus aureus,Bacillus subtilis,Escherichia coli,Candida albicans,Aspergillus niger and Penicillium funiculosum using cylinder-plate method.The results show that 12 strains have antimicrobial activity,accounting for 16.7% of the total,in which 7 strains are Bacillus spp.(accounting for 9.7%) and 5 strains are Gram-negative rod-shaped bacteria(accounting for 6.9%).The effects of antimicrobial activity produced by the antagonistic bacteria strains on the tested microorganisms are in the order of Gram-positive bacteria>Gram-negative bacteria>filamentous fungi,and antimicrobial activity of marine spore-producing bacteria is stronger than that of the Gram-negative bacteria.The mean isolation rate of the marine antagonistic bacteria strains with antimicrobial activities from the mangrove samples is the highest,up to 25.3%.The strain NB7.2 with strong antimicrobial activity is identified as Bacillus subtilis according to its morphological,physiological and biochemical characteristics,and 16S rRNA sequence analysis. |
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| Keywords: | marine bacteria antimicrobial activity identification of strain |
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