| 泥蚶锰超氧化物歧化酶基因的研究 |
| |
| 引用本文： | 杜丽丽,李太武,苏秀榕,李登峰,潘沙芳.泥蚶锰超氧化物歧化酶基因的研究[J].海洋科学集刊,2009,49(49):115-118. |
| |
| 作者姓名： | 杜丽丽 李太武 苏秀榕 李登峰 潘沙芳 |
| |
| 作者单位： | 宁波大学生命科学与生物工程学院;宁波大学生命科学与生物工程学院;宁波大学生命科学与生物工程学院;宁波大学生命科学与生物工程学院;宁波大学生命科学与生物工程学院 |
| |
| 摘 要： |
|
| 收稿时间： | 2007/10/25 0:00:00 |
Cloning and Analysis of Core Sequence of Tegillarca Granosa Mn-Superoxide Dismutase Gene |
| |
| Du Lili,Li Taiwu,Su Xiurong,Li Dengfeng and Pan Safang.Cloning and Analysis of Core Sequence of Tegillarca Granosa Mn-Superoxide Dismutase Gene[J].Studia Marina Sinica,2009,49(49):115-118. |
| |
| Authors: | Du Lili Li Taiwu Su Xiurong Li Dengfeng and Pan Safang |
| |
| Institution: | Faculty of Life Science and Biotechnology,Ningbo Uciversity;Faculty of Life Science and Biotechnology,Ningbo Uciversity;Faculty of Life Science and Biotechnology,Ningbo Uciversity;Faculty of Life Science and Biotechnology,Ningbo Uciversity;Faculty of Life Science and Biotechnology,Ningbo Uciversity |
| |
| Abstract: | Special primers were designed based on the sequence information of superoxide dismutase genes reported in the Iiterature and were used for RT-PCR and rapid amplification of cDNA ends(RACE) to clone Tegillarca granosaMn-superoxide dismutase gene successfully.The length cDNA of Tegillarca granosaMn-superoxide dismutase gene was of 635bp by including a 3'untranslated region(UTR)of 230bp and an open reading frame(ORF) of 4.5bp encoding a polypeptide of 135 amino acids. TheTegillarca granosaMn-superoxide dismutase gene shares 66%~73% homology with Mizuhopecteb yessoensis's,Danio rerio's,Xenopus Laevis's,Human's,Callinectes sapidus'sSOD CDS, while the corresponding amino acids shares 68%-78% homology and 80%~89% comparability. These indicate the SOD gene is high evolutional conservation in animals. |
| |
| Keywords: | |
|
| 点击此处可从《海洋科学集刊》浏览原始摘要信息 |
| 点击此处可从《海洋科学集刊》下载免费的PDF全文 |
|
