| 光照强度对海洋微藻脂肪含量及脂肪酸组成影响的研究 |
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| 引用本文： | 李荷芳,周汉秋.光照强度对海洋微藻脂肪含量及脂肪酸组成影响的研究[J].海洋科学集刊,2001(43):178-183. |
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| 作者姓名： | 李荷芳 周汉秋 |
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| 作者单位： | 中国科学院海洋研究所 |
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| 基金项目： | 中国科学院海洋研究所调查研究报告第4011号。国家自然科学基金资助项目，39570084号；国家“九五”攻关项目，96-916-04-03号。 |
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| 摘 要： | 近年来，海洋微藻脂肪酸组成的研究及其应用越来越受到国内外科学家的重视。高度不饱和脂肪酸(PUFA)，特别是长链的n-3 PUFA，如二十碳五烯酸(EPA)和二十二碳六烯酸(DHA)对海洋动物和人类都具有营养学和医学上的价值。在海洋微藻的培养过程中，光照强度是海洋微藻生长的条件之一，光线的明与暗，光度的强与弱，不仅对微藻的生长速率、产量有影响，而且对其脂肪含量和脂肪酸的组成也有影响(Teshima et al.，1983；Thompson et al.，1990； Renaud et al., 1991)。
作者在以往研究的基础上，选择了3种有代表性的海洋微藻：(1)小球藻Chlorella sp-2(李荷芳等，1999)，此藻脂肪酸中EPA含量高、且不含DHA；(2)球等鞭金藻(Isochrysis galbana)，该藻DHA含量较高，但几乎不含EPA；(3)前沟藻(Amphidinium sp.)的EPA、DHA含量均高。将以上3种微藻作为原料，在不同的光照强度下进行培养，测定并分析藻体中的脂肪含量和脂肪酸组成的变化，从而了解光强对海洋微藻脂肪含量及其脂肪酸组成的影响。
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| 关 键 词： | 海洋微藻 脂肪含量 光照强度 |
| 收稿时间： | 2000/3/30 0:00:00 |
EFFECTS OF LIGHT INTENSITY ON THE CONTENT OF TOTAL LIPID AND FATTY ACID COMPOSITION OF MARINE MICROALGAE |
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| Li Hefang,Zhou Hanqiu.EFFECTS OF LIGHT INTENSITY ON THE CONTENT OF TOTAL LIPID AND FATTY ACID COMPOSITION OF MARINE MICROALGAE[J].Studia Marina Sinica,2001(43):178-183. |
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| Authors: | Li Hefang Zhou Hanqiu |
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| Institution: | Institute of Oceanology, Chinese Academy of Sciences |
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| Abstract: | This study on the effect of 12L: 12D cycle and continuous light intensity (8000 lx, 4000 lx, 2000 lx 12h: 12h L D cycle and 4000 lx) on the total lipid content and fatty acid composition of three species of marine microalgae (Chlorella sp-2, Isochrysis galbana, and Amphidinium sp.) showed that the major fatty acids of Chlorella sp-2 were 16: 0, 16:1 (n-7) and 20:5 (n-3); those of Isochrysis galbana were 14:0, 16:0, 16:1(n-7), 18:4(n-3), and 22:6(n-3); and those of Amphidinium sp. Were 16:0, 18:1(n-9),18:4(n-3), 20:5 (n-3) and 22:6 (n-3). The total lipid contents of Chlorella sp-2 and Isochrysis galbana grown at higher light intensity were higher than those grown at lower light intensity. Generally, their percentage of the saturated and monounsaturated fatty acids increased with light intensity. The total polyunsaturated fatty acids decreased with increasing light intensity. EPA content decreased with increasing light intensity and the was no obvious changes in DHA as light intensity increased. |
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| Keywords: | marine microalgae total lipid content light intensity |
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