| 养殖大菱鲆烂鳍病病原菌的鉴定及系统发育学研究 |
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| 引用本文： | 张正,王印庚,韩文君,李秋芬.养殖大菱鲆烂鳍病病原菌的鉴定及系统发育学研究[J].海洋科学进展,2004,22(2):193-197. |
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| 作者姓名： | 张正 王印庚 韩文君 李秋芬 |
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| 作者单位： | 1. 中国海洋大学,海洋生命学院,山东,青岛,266003
2. 中国水产科学院,黄海水产研究所,山东,青岛,266071 |
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| 基金项目： | 国家高新技术研究发展计划项目——工厂化养殖大菱鲆疾病综合控制技术及中试示范(2001AA622070) |
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| 摘 要： | 从山东半岛3个养殖场各分离到一株大菱鲆烂鳍病的病原菌，均为革兰氏阴性，短杆状，极生鞭毛，有运动能力，菌落半透明。经常规生理生化特征测试和16SrRNA基因序列对比，表明它们都是同一种细菌。理化特征的结果显示这3株菌均与V．anguillarum的表性特征非常相似。对其16SrRNA基因序列进行分析，并构建了系统发育树，结果表明这些菌株与V．anguillarum亲缘关系最近。综合上述研究结果，将该菌鉴定为V．anguillarum.
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| 关 键 词： | 大菱鲆 烂鳍 16S rRNA 系统发育树 Vibrio anguillarum |
| 文章编号： | 1671-6647(2004)02-0193-05 |
| 修稿时间： | 2003年12月9日 |
Identification and Phylogenetic Study of Pathogenic Bacteria Causing Ulcerative Fin of Cultured Turbot Scophthalmus maximus |
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| ZHANG Zheng.Identification and Phylogenetic Study of Pathogenic Bacteria Causing Ulcerative Fin of Cultured Turbot Scophthalmus maximus[J].Advances in Marine Science,2004,22(2):193-197. |
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| Authors: | ZHANG Zheng |
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| Institution: | ZHANG Zheng~ |
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| Abstract: | Three strains of pathogenic bacteria causing ulcerative fin of turbot were isolated from three fish farms of Shadong Peninsula, and they are all Gram-negative, rod-shaped and motile with polar flagella and translucent colony. It is shown from the conventional physiological and biochemical test and the 16S ribosomal RNA sequencing contrast results that they are the same species of bacterium, and their characteristics are very similar to those of Vibrio anguillarum. Their 16S ribosomal RNA gene sequences were analyzed, their phylogenetic dendrograms were constructed, and it is shown from the anlysis results that these bacterial strains have a close phylogenetic relation to Vibrio anguillarum, so the pathogenic bacteria are (identified) as Vibrio anguillarum. |
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| Keywords: | Scophthalmus maximus ulcerative fin 16S ribosomal RNA phylogenetic dendrogram Vibrio anguillarum |
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