| 南极放线菌NJ-F2萃取物的抗菌活性初步研究 |
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| 引用本文： | 李江,林学政,沈继红,王能飞,李光友.南极放线菌NJ-F2萃取物的抗菌活性初步研究[J].海洋科学进展,2009,27(4). |
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| 作者姓名： | 李江 林学政 沈继红 王能飞 李光友 |
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| 作者单位： | 国家海洋局,第一海洋研究所海洋生态研究中心,山东,青岛,266061 |
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| 基金项目： | 国家海洋局第一海洋研究所基本科研业务费--极地海洋放线菌抗生素的筛选，国家高技术研究发展计划重点项目--极地特殊海洋微生物资源利用的关键技术研究 |
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| 摘 要： | 从南极第24次科学考察采集的海泥样品中分离获得放线菌NJ-F2,对NJ-F2发酵液的乙酸乙酯萃取物进行体外抗菌实验,并采用16S rDNA方法对NJ-F2进行系统发育分析.结果表明萃取物对金黄色葡萄球菌和枯草芽孢杆菌的最低抑菌浓度(MIC)、最低杀菌浓度(MBC)分别为0.25 mg/mL,5.0 mg/mL;而对大肠杆菌和产气杆菌的MIC、MBC分别为0.5 mg/mL,5.0 mg/mL,但对真菌没有抗菌作用;16SrDNA分析结果表明NJ-F2为链霉菌属(Streptomyces sp.).
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| 关 键 词： | 南极放线菌Streptomyces sp.NJ-F2 萃取物 抗菌 发育分析 |
Preliminary Study on Antibiotic Activity of the Extract From Antarctic Actinomycete NJ-F2 |
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| LI Jiang,LIN Xue-zheng,SHEN Ji-hong,WANG Neng-fei,LI Guang-you.Preliminary Study on Antibiotic Activity of the Extract From Antarctic Actinomycete NJ-F2[J].Advances in Marine Science,2009,27(4). |
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| Authors: | LI Jiang LIN Xue-zheng SHEN Ji-hong WANG Neng-fei LI Guang-you |
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| Institution: | LI Jiang,LIN Xue-zheng,WANG Neng-fei,LI Guang-you(Research Center for Marine Ecology,First Institute of Oceanorgarphy,SOA,Qingdao 266061,China) |
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| Abstract: | Actinomycete NJ-F2 is isolated from the sea-mud sampled during the 24th Antarctic Scientific Expedition of China . The bacteriostatic activity of the extract with acetic ether from the fermented NJ-F2 is detected in the in-vitro experiment and the 16S rDNA method is employed to analyze the NJ-F2 phylogenesis. As it is shown from the experimental results,the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) against both Staphylococcus aureus and Bacillus subtilis are 0. 25 mg/mL and 5. 0 mg/mL respectively,and MIC and MBC against both Eschericidal coil and Aerobac-tor aerogenes are 0. 5 mg/mL and 5. 0 mg/mL respectively. No antibacterial activity can be found against fungi. It is learnt from the 16S rDNA analysis that NJ-F2 is of Streptomyces sp. |
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| Keywords: | Antarctic actinomycete Streptomyces sp NJ-F2 extract antibiotic activity phylogenetic analysis |
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