| 银鲳应对高温胁迫的生理响应及其相关基因表达研究 |
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| 引用本文： | 史琛榆,赵淳朴,胡艺潇,沙桃平,王亚军,王丹丽,徐善良.银鲳应对高温胁迫的生理响应及其相关基因表达研究[J].应用海洋学学报,2022,41(1):1-7. |
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| 作者姓名： | 史琛榆 赵淳朴 胡艺潇 沙桃平 王亚军 王丹丽 徐善良 |
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| 作者单位： | 宁波大学海洋学院，浙江 宁波 315211;宁波大学应用海洋生物技术教育部重点实验室，浙江 宁波 315211 |
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| 基金项目： | 国家自然科学基金资助项目(31872586)； 浙江省自然科学基金资助项目(LY18C190008)；浙江省重大科技专项资助项目(2019C02059) |
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| 摘 要： | 为探究银鲳(Pampus argenteus)应对高温胁迫的生理响应及其相关基因表达研究,本研究设置4个温度组:对照组26℃和实验组28、30、32℃.并对银鲳的肝、肾和鳃的组织结构、肝组织中超氧化物歧化酶、过氧化氢酶的相对活性变化和丙二醛含量变化以及hsp70基因的相对表达量的变化进行了研究.结果显示:(1)随温度的...
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| 关 键 词： | 海洋生物学 银鲳 高温胁迫 组织损伤 酶活性 hsp70基因表达 |
Effects of high temperature stress on physiology and expression of related gene in Pampus argenteus |
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| SHI Chenyu,ZHAO Chunpu,HU Yixiao,SHA Taoping,WANG Yajun,WANG Danli,XU Shanliang.Effects of high temperature stress on physiology and expression of related gene in Pampus argenteus[J].Journal of Applied of Oceanography,2022,41(1):1-7. |
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| Authors: | SHI Chenyu ZHAO Chunpu HU Yixiao SHA Taoping WANG Yajun WANG Danli XU Shanliang |
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| Institution: | (School of Marine Sciences, Ningbo University, Ningbo 315211, China;Key Laboratory of Applied Marine Biotechnology, Ministry of Education, Ningbo University, Ningbo 315211, China) |
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| Abstract: | To investigate the physiological responses and related gene expression of Pampus argenteus under high temperature stress,four temperature treatments,control group(26℃)and experimental groups(28,30,32℃),were set up for the experiment.The changes of tissue structure in liver,kidney and gill,the enzyme activities of superoxide dismutase(SOD)and catalase(CAT),the content of malondialdehyde(MDA)and the relative expression level of hsp70 gene were studied.The results showed that(1)with increasing temperature,the tissue structure damaged gradually and worsened,particularly for the tissues of liver and gill.(2)The high temperature significantly affected the antioxidant activity of the fish showing irreversible damage at 30℃and 32℃except for the treatment of 28℃where the fish was getting adapted.(3)When the stress extended,the expression levels of hsp70 gene in all treatments increased significantly at first and reached a peak in 0.5 d and then decreased quickly.It shows that under short-term heat stress,P.argenteus gradually adapted to 28℃while under high temperature stress of 30℃and 32℃irreversible and deadly damage occurred in the fish body. |
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| Keywords: | marine biology Pampus argenteus high temperature stress tissue damage enzyme activity hsp 70 gene expression level |
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