| 海洋来源白浅灰链霉菌中环二肽类化合物的分离与结构鉴定 |
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| 引用本文： | 古静燕,刘红兵,崔承彬,顾谦群.海洋来源白浅灰链霉菌中环二肽类化合物的分离与结构鉴定[J].中国海洋大学学报(自然科学版),2005,35(4):618-620. |
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| 作者姓名： | 古静燕 刘红兵 崔承彬 顾谦群 |
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| 作者单位： | 1. 中国海洋大学海洋药物教育部重点实验室,海洋药物与食品研究所,山东,青岛,266003
2. 中国海洋大学海洋药物教育部重点实验室,海洋药物与食品研究所,山东,青岛,266003;军事医学科学院毒物药物研究所,北京,100850 |
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| 基金项目： | 国家高技术研究发展计划项目(2003AA624020,2001A624020)，国家杰出青年科学基金(39825126)，国家自然科学基金(30300433)，山东省科技攻关计划(0121100107)，山东省自然科学基金重点(Z2001C01)，国家教育部长江学者奖励计划基金资助 |
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| 摘 要： | 为了寻找新的抗肿瘤活性化合物，采用硅胶、Sephadex LH-20柱色谱和高效液相色谱，从白浅灰链霉菌Streptomyces albogriseolus(A2002)中分离得到3个环二肽；利用理化及波谱学方法鉴定其结构分别为环(丙氨酸-缬氨酸)cyclo-(Ala-Val)，1]，环(丙氨酸-脯氨酸)cyclo-(Pro-Ala)，2]和环(脯氨酸-酪氨酸)cyclo-(Pro-Tyr)，3]。
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| 关 键 词： | 链霉菌 结构鉴定 Streptomyces 分离 Sephadex 肽类 海洋 高效液相色谱 活性化合物 谱学方法 丙氨酸 脯氨酸 抗肿瘤 柱色谱 环二肽 缬氨酸 酪氨酸 |
| 文章编号： | 1672-5174(2005)04-618-03 |
| 修稿时间： | 2004年11月30 |
Isolation and Identification of Cyclic Dipeptides from a Marine-Derived Streptomyces albogriseolus |
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| GU Jing-Yan,LIU Hong-Bing,CUI Cheng-bin,GU Qian-qun.Isolation and Identification of Cyclic Dipeptides from a Marine-Derived Streptomyces albogriseolus[J].Periodical of Ocean University of China,2005,35(4):618-620. |
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| Authors: | GU Jing-Yan LIU Hong-Bing CUI Cheng-bin GU Qian-qun |
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| Abstract: | In the course of our screening for new anti-tumor compounds from marine-derived microbial strains, it was found that the acetonic extract from the fermentation of Streptomyces albogriseolus A2002, a marine-derived actinomycete strain isolated from a Jiaozhou Bay sediment sample, significantly inhibited the cell cycle of tsFT210 cells at the G2/M phase and induced apoptosis in tsFT210 cells. Thus, the chemical examination was undertaken on the constituents of bioactive fractions obtained by bioassay-guided fractionation from the fermentation of producing strains. Compounds 1-3 were isolated by repeated column chromatography over Sephadex LH-20 and silica gel, followed by the prepared HPLC separation. Their structures were identified as cyclic dipeptides, cyclo-(Ala-Val) (1), cyclo-(Ala-Pro) (2) and cyclo-(Pro-Tyr) (3), respectively, by the spectroscopic method. |
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| Keywords: | marine-derived microorganism Streptomyces albogriseolus cyclic dipeptides chromatography separation |
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