| 检测海洋弧菌的酶联免疫吸附试验研究 |
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| 引用本文： | 张晓华,徐怀恕.检测海洋弧菌的酶联免疫吸附试验研究[J].中国海洋大学学报(自然科学版),1997(3). |
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| 作者姓名： | 张晓华 徐怀恕 |
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| 作者单位： | 青岛海洋大学海洋生命学院 |
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| 摘 要： | 研究建立中国对虾病原菌┐副溶血弧菌（Ｖｉｂｒｉｏｐａｒａｈａｅｍｏｌｙｔｉｃｕｓ）的间接酶联免疫吸附试验（ＥＬＩＳＡ）快速检测技术。间接ＥＬＩＳＡ技术中的副溶血弧菌的特异抗血清由家兔制备，羊抗兔ＩｇＧ用碱性磷酸酶标记，酶的底物为对磷酸基硝基苯。将该技术标准化后，测定了抗血清与１３株其它副溶血弧菌菌株及２９株其它弧菌标准菌株的交叉反应，并进行了苗期对虾样品中副溶血弧菌的检测
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| 关 键 词： | 海洋弧菌 间接酶联免疫吸附试验 快速检测技术 中国对虾 副溶血弧菌 |
AN INDIRECT ENZYME LINKED IMMUNOSORBENT ASSAY FOR RAPID DIAGNOSIS OF MARINE VIBRIO sp. |
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| Zhang XiaoHua,Peter Robertson,Brian Austin,Xu HuaiShu.AN INDIRECT ENZYME LINKED IMMUNOSORBENT ASSAY FOR RAPID DIAGNOSIS OF MARINE VIBRIO sp.[J].Periodical of Ocean University of China,1997(3). |
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| Authors: | Zhang XiaoHua Peter Robertson Brian Austin Xu HuaiShu |
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| Institution: | Zhang XiaoHua 1 Peter Robertson 2 Brian Austin 2 Xu HuaiShu |
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| Abstract: | An indirect Enzyme Linked Immunosorbent Assay (ELISA) for rapid diagnosis of Vibrio parahaemolyticus has been developed. Antiserum specific for the Vibrio parahaemolyticus was produced from rabbits and used in an indirect ELISA incorporating Alkaline Phosphatase conjugated anti rabbit IgG goat serum, and p Nitrophenyplosphate (pNPP) as substrate. After the method was optimized, cross reactions of antisera with 13 strains of V. parahaemolyticus and type strains of vibrio were detected. |
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