| 仿刺参体腔细胞原代培养以及灿烂弧菌胞外产物对其毒性的研究 |
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| 引用本文： | 许乐乐,麦康森,马洪明,张文兵.仿刺参体腔细胞原代培养以及灿烂弧菌胞外产物对其毒性的研究[J].中国海洋大学学报(自然科学版),2010,40(3). |
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| 作者姓名： | 许乐乐 麦康森 马洪明 张文兵 |
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| 作者单位： | 中国海洋大学教育部海水养殖重点实验室,山东,青岛,266003 |
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| 基金项目： | 国家高技术研究发展计划项目 |
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| 摘 要： | 采用CCM、HSM和L-15N 3种培养液对刺参(Apostichopus japonicas)的体腔细胞进行了体外原代培养,并用MTT还原法对细胞的体外存活力进行测定,结果显示:CCM和HSM培养的体腔细胞,在体外分别存活至3 d和6 d大量死亡;L-15N培养液可使细胞在1周内保持存活.用刺参病原菌灿烂弧菌的胞外产物与刺参体外培养细胞共培育,发现对体腔细胞有毒性作用,半致死蛋白质浓度(IC_(50))为27.6 μg/mL.
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| 关 键 词： | 刺参 体腔细胞 体外细胞培养 灿烂弧菌 胞外产物 |
Culture of Coeleomocytes of Sea Cucumber,Apostichopus japonicas in Vitro and the Cytotoxicity Effect of Extracellular Products of Vibrio splendidus to the Coelomocytes |
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| XU Le-Le,MAI Kang-Sen,MA Hong-Ming,ZHANG Wen-Bing.Culture of Coeleomocytes of Sea Cucumber,Apostichopus japonicas in Vitro and the Cytotoxicity Effect of Extracellular Products of Vibrio splendidus to the Coelomocytes[J].Periodical of Ocean University of China,2010,40(3). |
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| Authors: | XU Le-Le MAI Kang-Sen MA Hong-Ming ZHANG Wen-Bing |
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| Institution: | XU+Le-Le,MAI+Kang-Sen,MA+Hong-Ming,ZHANG+Wen-Bing+(The+Key+Laboratory+of+Mariculture,Ministry+of+Education,Ocean+University+of+China,Qingdao+266003,China) |
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| Abstract: | The coelomocytes of sea cucumber,Apostichopus japonicas,was cultured in vitro using three different culture mediums,namely CCM,HSM,L-15N,and the MTT reduction method was employed to detect the cells viability in these mediums.The coelomocytes viability of sea cucumber decreased significantly at day 3 and day 6 after being cultured in vitro with CCM and HSM respectively.However,L-15N medium could keep coelomocytes alive stably in vitro for at least one week,without significant viability loss.Extracellular pr... |
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| Keywords: | sea cucumber Apostichopus japonicas coelomocytes in vitro cell culture Vibrio splendisus extracellular products |
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