| 青岛地区近岸海水和几种海产品中付溶血性弧菌的调查分析(英文) |
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| 引用本文： | 陈世阳,刘树青,张文芳.青岛地区近岸海水和几种海产品中付溶血性弧菌的调查分析(英文)[J].中国海洋大学学报(自然科学版),1991(1). |
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| 作者姓名： | 陈世阳 刘树青 张文芳 |
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| 作者单位： | 青岛海洋大学食品工程系
(陈世阳，刘树青)，青岛海洋大学食品工程系(张文芳) |
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| 摘 要： | 付溶血性弧菌(Vibrio parahaemolyticus)是水产品中的一种重要食物中毒菌,它在沿岸海水和海产动物体上都有广泛的分布。本文报告了青岛地区近岸海水和几种常见水产品污染了该菌的情况。在检测方法上,改进了一种选择性较强的培养基(SAC),经实验表明,SAC培养基较其他常用的几种培养基对付溶血性弧菌检出的阳性率和灵敏度均较高,与普通采用的TCBS法比较,x~2=4.28,ρ<0.05,有显著效果的意义。青岛近岸海水中付溶血性弧菌的平均出现率为63.25—463CFU/100ml,生鲜的水产食品中平均含有745～6900CFU/100g,其中贝类含量最多,季节性和海区对其检出率有明显影响,夏季检出率最高,冬季最低,对不同方法所检出的菌株,选出部分作了它们的主要生理、生化特性的鉴定,并与参考菌株对比,证实SAC法检出的菌株有85％以上的株数符合付溶血性弧菌的特征,而TCBS法所检出菌株只有55％的真实性。并初步证明,这是一种温血动物和冷血动物的共患菌。
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| 关 键 词： | 付溶血性弧菌 海产品食物中毒 选择性培养基 |
OCCURRENCE OF VIBRIO PARAHAEMOLYTICUS IN SEAWATER AND SOME SEAFOODS IN THE COASTAL AREA OF QINGDAO |
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| Chen Shiyang Liu Shuqing Zhang Lifang.OCCURRENCE OF VIBRIO PARAHAEMOLYTICUS IN SEAWATER AND SOME SEAFOODS IN THE COASTAL AREA OF QINGDAO[J].Periodical of Ocean University of China,1991(1). |
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| Authors: | Chen Shiyang Liu Shuqing Zhang Lifang |
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| Institution: | Department of Food Technology |
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| Abstract: | A selective agar medium was developed in this investigation and designated as the "SAC" medium.Because the majority of the Vibrio parahaemolyticus are halophile and utilize arabi-nose as a carbon source to produce typical yellow colonies on the SAC medium,whereas most of closely related species can produce only greenish minute colonies,the growth of usual marine microflora can be inhibited.Using this method,the numbers of organisms in coastal seavvater and some fresh seafoods were determined.It was revealed that the average counts of Vibrio parahaemolyticus in seawatcr ranged from 63-463 CFU per 100 ml and 745-6900 CFU per 100 grams in seafoods;clams contained the highest number than others.A certain numbers of isolates were tested;approximately 85 percent of the isolates were confirmed to be V.parahaemolyticus. |
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| Keywords: | Vibro parahaemolyticus seafood poisoning selective agar medium |
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