| 雄性半滑舌鳎雄激素受体基因的克隆与表达分析 |
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| 引用本文： | 温海深,张葭人,陈彩芳,金国雄,陈晓燕,史宝,何峰.雄性半滑舌鳎雄激素受体基因的克隆与表达分析[J].中国海洋大学学报(自然科学版),2009,39(3). |
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| 作者姓名： | 温海深 张葭人 陈彩芳 金国雄 陈晓燕 史宝 何峰 |
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| 作者单位： | 中国海洋大学海水养殖教育部重点实验室,山东,青岛,266003 |
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| 摘 要： | 利用RT-PCR方法从半滑舌鳎(Cynoglossus semilaevis)中克隆了雄激素受体(Androgen receptor,AR)基因cDNA的部分序列(434 bp),经BLAST比对与狼鲈(Dicentrarchus labrax)、花溪(Kryptolebias marmoratus)、三斑海猪鱼(Halichoeres trimaculatus)和三刺鱼(Gasterosteus aculeatus)的雄激素受体同源性分别为84%,82%,82%和81%.组织表达分析表明,AR基因在半滑舌鳎的性腺、肝、胃、脾、肾、头肾、肠、鳃、心、脑和肌肉这11种组织中均有表达,表达量有所差异.肾中表达量最丰富,鳃中最少.根据其他硬骨鱼类表达模式,推测半滑舌鳎可能只含有1种AR.
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| 关 键 词： | 半滑舌鳎 雄激素受体 克隆 表达 |
Cloning and Expression Analysis of Male Half-Smooth Tongue Sole(Cynoglossus semilaevis)'s Androgen Receptor Gene |
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| WEN Hai-Shen,ZHANG Jia-Ren,CHEN Cai-Fang,JIN Guo-Xiong,CHEN Xiao-Yan,SHI Bao,HE Feng.Cloning and Expression Analysis of Male Half-Smooth Tongue Sole(Cynoglossus semilaevis)''s Androgen Receptor Gene[J].Periodical of Ocean University of China,2009,39(3). |
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| Authors: | WEN Hai-Shen ZHANG Jia-Ren CHEN Cai-Fang JIN Guo-Xiong CHEN Xiao-Yan SHI Bao HE Feng |
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| Institution: | The Key Laboratory of Mariculture;Ministry of Education;Ocean University of China;Qingdao 266003;China |
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| Abstract: | Partial androgen receptor(AR) gene(454 bp in length) had been cloned from half-smooth tongue sole(Cynoglossus semilaevis) by RT-PCR method.Alignment analysis showed that the sequence was 84%,82%,82% and 81% similar to those of sea bass,mangrove rivulus,tripletailed wrasse and threespine stickleback,respectively.Tissue expression analysis showed that AR mRNA expressed in all eleven tissues including gonad,liver,stomach,spleen,kidney,head kidney,intestines,gill,heart,brain and muscle,the highest expression le... |
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| Keywords: | half-smooth tongue sole AR clone expression |
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