| 碱湖高产碱性蛋白酶菌的选育和产酶条件研究 |
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| 引用本文： | 梅承芳,江晓路,牟海津,王鹏.碱湖高产碱性蛋白酶菌的选育和产酶条件研究[J].中国海洋大学学报(自然科学版),2005,35(4):613-617. |
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| 作者姓名： | 梅承芳 江晓路 牟海津 王鹏 |
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| 作者单位： | 中国海洋大学生命科学与技术学部,山东,青岛,266003 |
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| 摘 要： | 以分离自碱湖的产碱性蛋白酶菌V．metschnikovii DL33为出发株，经紫外线、硫酸二乙酯多次复合诱变，获得一高产突变株V．Metschnikovii DL33—51，其产生的碱性蛋白酶活力是出发菌株的5．74倍，48h发酵液酶活由350U／mL提高至2010U／mL。同时对菌株DL33—51的产酶培养基和条件进行了优化，以最适产酶培养基26℃对V．metschnikovii DL33—51进行摇瓶发酵，发酵40h，酶活达3268U／mL。
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| 关 键 词： | 碱湖 碱性蛋白酶 V．metschnikovii DL33-51 |
| 文章编号： | 1672-5174(2005)04-613-05 |
| 修稿时间： | 2004年2月24日 |
Screening of Alkaline Protease-Producing Banterial Strais Isolated from a Soda Lake and Their Fermentation Conditions |
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| MEI Cheng-Fang,JIANG Xiao-lu,MU Hai-Jin,WANG Peng.Screening of Alkaline Protease-Producing Banterial Strais Isolated from a Soda Lake and Their Fermentation Conditions[J].Periodical of Ocean University of China,2005,35(4):613-617. |
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| Authors: | MEI Cheng-Fang JIANG Xiao-lu MU Hai-Jin WANG Peng |
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| Abstract: | An alkaline protease producer DL33 was isolated from a soda lake and identified as Vibrio metschnikovii. By combination treatment of DL33 with UV and DES, a mutant DL33-51 was obtained, whose enzyme activity was 5.74 times that of the original strain, reaching to 2010U/mL with 48h shaking flask. The condition of enzyme production under flask shaking were investigated. Under the optimum condition, the alkaline protease activity could reach to 3 268U/mL, with 40h shaking flask. |
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| Keywords: | soda lake alkaline protease V metschnikovii DL33-51 |
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