| 栉孔扇贝DAPI带型和PI带型研究 |
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| 引用本文： | 徐俊,包振民,任晓亮,王珊,胡丽萍,黄晓婷.栉孔扇贝DAPI带型和PI带型研究[J].中国海洋大学学报(自然科学版),2011,41(4):77-80,102. |
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| 作者姓名： | 徐俊 包振民 任晓亮 王珊 胡丽萍 黄晓婷 |
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| 作者单位： | 1. 中国海洋大学海洋生物遗传育种教育部重点实验室,山东青岛266003;上海市医药学校,上海200135
2. 中国海洋大学海洋生物遗传育种教育部重点实验室,山东青岛,266003 |
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| 基金项目： | 国家高技术研究发展计划项目(2006AA10A408,2010AA10A401); 国家自然科学青年基金项目(30901096); 中国科学院实验海洋生物学重点实验室开放基金项目(KFN92007NO12)资助 |
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| 摘 要： | 选取栉孔扇贝Chlamys farreri担轮幼虫为材料,采用秋水仙素-低渗-空气干燥法制备染色体标本,应用荧光显带技术,分析了DAPI带和PI带在栉孔扇贝染色体上的分布。DAPI带型结果显示,栉孔扇贝所有染色体上都存在DAPI阳性带,主要分布于传统的着丝粒区和端部区域,另外还存在一些中间区DAPI带及可变带,总带数为62。PI带型结果与DAPI带型结果相似,在所有染色体上都存在PI阳性带。2种带型的阳性带所在位置与异染色质分布区域相吻合。
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| 关 键 词： | 栉孔扇贝(Chlamys farreri) 染色体 DAPI带 PI带 |
DAPI-Banding and PI-Banding of Zhikong Scallop (Chlamys farreri) |
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| XU Jun,BAO Zhen-Min,REN Xiao-Liang,WANG Shan,HU Li-Ping,HUANG Xiao-Ting.DAPI-Banding and PI-Banding of Zhikong Scallop (Chlamys farreri)[J].Periodical of Ocean University of China,2011,41(4):77-80,102. |
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| Authors: | XU Jun BAO Zhen-Min REN Xiao-Liang WANG Shan HU Li-Ping HUANG Xiao-Ting |
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| Institution: | XU Jun1,2,BAO Zhen-Min1,REN Xiao-Liang1,WANG Shan1,HU Li-Ping1,HUANG Xiao-Ting1(1.Key Laboratory of Marine Genetics and Breeding,Ministry of Education,Ocean University of China,Qingdao 266003,China,2.Shanghai Pharmaceutical School,Shanghai 200135,China) |
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| Abstract: | In this paper,the techniques including DAPI-staining and PI-staining were used to study the DAPI-banding and PI-banding patterns of Chlamys farreri.Chromosomes spread from trochophore were prepared by Colchine-hypotonic-air drying methods.The results showed that DAPI-bands displayed in all the chromosomes of C.farreri,and they were mostly distributed in centromeric regions and terminal regions.Some interstitial bands and variable bands were also observed.62 DAPI positive bands were recorded in 12 chromosome... |
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| Keywords: | Zhikong scallop(Chlamys farreri) chromosome DAPI-banding PI-banding |
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