首页 | 本学科首页   官方微博 | 高级检索  
     检索      

岩藻聚糖硫酸酯低聚糖的制备及其抗氧化活性研究
引用本文:董平,薛长湖,李兆杰,徐杰,赵雪,蔡跃飘,张永勤.岩藻聚糖硫酸酯低聚糖的制备及其抗氧化活性研究[J].中国海洋大学学报(自然科学版),2006,36(Z1):59-62.
作者姓名:董平  薛长湖  李兆杰  徐杰  赵雪  蔡跃飘  张永勤
作者单位:中国海洋大学食品科学与工程学院,山东,青岛,266003
基金项目:高比容电子铝箔的研究开发与应用项目
摘    要:探讨H2O2降解岩藻聚糖硫酸酯的条件及其产物的抗氧化活性。采用Cu2 -H2O2体系降解原料Fucoidan,结果发现反应时间越长得到的高分子量的组分越少,而低分子量的低聚糖产量增加;H2O2浓度增高,水解所得主要组分的分子量明显减小;温度升高,水解速度明显加快;产物的硫酸根含量随反应温度和H2O2浓度的降低而增加。用Pyrogallol—Luminol系统对水解产物的抗氧化活性进行了研究,发现硫酸根含量低的Fucoidan水解产物除O2.-的活性较好,其中15%H2O2降解得<5KD的低聚糖清除O2.-的活性明显高于肌肽(IC50为0.65mg/mL),IC50为0.17mg/mL。

关 键 词:岩藻聚糖硫酸酯  H2O2降解  抗氧化活性
文章编号:1672-5174(2006)03Ⅱ-059-04
修稿时间:2005年4月28日

Preparation and Antioxidative Activity of Low Molecular Weight Fucoidan
DONG Ping,XUE Chang-Hu,LI Zhao-Jie,XU Jie,ZHAO Xue,CAI Yue-Piao,ZHANG Yong-Qin.Preparation and Antioxidative Activity of Low Molecular Weight Fucoidan[J].Periodical of Ocean University of China,2006,36(Z1):59-62.
Authors:DONG Ping  XUE Chang-Hu  LI Zhao-Jie  XU Jie  ZHAO Xue  CAI Yue-Piao  ZHANG Yong-Qin
Abstract:The conditions of fucoidan degradation by H_2O_2 and the antioxidative activities of degradated products studied.The raw fucoidan was degradated by a Cu~(2 )-H_2O_2 assay,the results showed that the percentage of the products of high molecular weight decreased with the reaction time;however,those of low molecular weight increased;the molecular weight of the hydrolysate showed a negative relationship with the concentration of H_2O_2;the reaction rate of degradation was enhanced marked with increasing temperature;and the SO~(2-)_4 content of the oligosaccharides increased with decreasing temperature and H_2O_2 concentration.The antioxidative activities of degradated products were assessed by the pyrogallcl-luminol system,the results showed that the O_2~(.-) scavenging ability of the degradated products of lower SO~(2-)_4 contents was better,the fraction with MW<5KD(degradated by 15% H_2O_2) had obvious higher O_2~(.-) scavenging ability than Sarcosine(IC_(50)=(0.65)mg/mL),whose IC_(50) was 0.17mg/mL.
Keywords:fucoidan  H_2O_2 hydrolysis  antioxidative activity
本文献已被 CNKI 万方数据 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号