| 青岛近海琼胶降解细菌的筛选和多样性分析 |
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| 引用本文： | 杜宗军,赵苑,李美菊,池振明,吴龙飞.青岛近海琼胶降解细菌的筛选和多样性分析[J].中国海洋大学学报(自然科学版),2007,37(2):277-282. |
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| 作者姓名： | 杜宗军 赵苑 李美菊 池振明 吴龙飞 |
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| 作者单位： | 1. 中国海洋大学生命科学与技术学部海洋生命学院,山东,青岛,266003
2. 中国海洋大学生命科学与技术学部海洋生命学院,山东,青岛,266003;法国科研中心马赛结构生物学-微生物学研究所细菌化学实验室,法国马赛 |
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| 基金项目： | 中国海洋大学绿卡人才工程科研启动基金 |
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| 摘 要： | 对青岛近海海水中琼胶降解细菌进行系统的分离,筛选得到87株海洋琼胶降解细菌。从中选出15株有代表性的菌株,克隆其16SrDNA序列,进行分子鉴定。结果表明,这些细菌主要分布在Cellulophaga,Cytophaga,Microbulbifer,Glaciecola,Pseudoalteromonas,Pseudomonas,Alteromonas和Agarivorans共8个属中。其中QM5,QM21,QM36鉴定为Cellulophaga lytica,QM15和QM28鉴定为Glaciecola mesophila,QM11,QM35,QM47,QM65分别鉴定为Cytophaga fuci-cola,Pseudoalteromonas atlantica,Pseudoalteromonas haloplanktis,Alteromonas addita。其余5株菌能够鉴定到属的水平。其中1株细菌QM42还不能确立分类地位。
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| 关 键 词： | 细菌多样性 琼胶降解细菌 琼胶酶 |
| 文章编号： | 1672-5174(2007)02-277-06 |
| 修稿时间： | 2006-09-04 |
Screening and Biodiversity Analysis of Agarolytic Bacteria in the Qingdao Coast |
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| Du Zong-Jun,ZHAO Yuan,LI Mei-Ju,CHI Zhen-Ming,WU Long-Fei.Screening and Biodiversity Analysis of Agarolytic Bacteria in the Qingdao Coast[J].Periodical of Ocean University of China,2007,37(2):277-282. |
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| Authors: | Du Zong-Jun ZHAO Yuan LI Mei-Ju CHI Zhen-Ming WU Long-Fei |
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| Institution: | 1. College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China; 2. Laboratoire de Chimie Bacterienne, Institute de la Biologie Structure et Microbiologie, CNRS Marseille, France |
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| Abstract: | A preliminary screening for agar-degrading bacteria from the coastal waters of Qingdao yielded 87 isolates with promising agarolytic activity,among which 15 strains were selected for further analysis.To investigate the phylogenetic position of these strains,the 16S rDNA sequences were cloned,sequenced and compared with those of related strains.A molecular phylogenetic dendrogram was constructed based on genetic distance analysis.The strains were classified as members of genera Cellulophaga,Cytophaga,Microbulbifer,Glaciecola,Pseudoalteromonas,Pseudomonas,Alteromonas and Agarivorans.Strains QM5,QM21 and QM36 were identified to be Cellulophaga lytica.Strains QM15 and QM28 were identified to be Glaciecola mesophila.Strains QM11,QM35,QM47 and QM65 were identified to be Cytophaga fucicola,Pseudoalteromonas atlantica,Pseudoalteromonas haloplanktis and Alteromonas addita,respectively. |
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| Keywords: | 16SrDNA |
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