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青岛近海碳钢锈层中细菌群落结构的多样性
引用本文:栾鑫,段继周,池振明.青岛近海碳钢锈层中细菌群落结构的多样性[J].中国海洋大学学报(自然科学版),2012(Z1):107-115.
作者姓名:栾鑫  段继周  池振明
作者单位:中国海洋大学海洋生命学院;中国科学院海洋研究所
基金项目:中国科学院重要方向性项目(KZCX2-EW-205);国家自然科学基金项目(40976046)资助
摘    要:研究海水环境中Q235碳钢锈层中微生物群落的多样性。应用16SrDNA基因文库技术,分别对实海全浸一年的Q235碳钢内外锈层中的微生物进行基因文库的构建和分析。通过克隆测序构建了含有64个OTUs的细菌16SrRNA基因文库,结果表明锈层中微生物种类丰富,主要分布于13个已知的菌门,包括变形菌门;拟杆菌门;硅藻门;酸杆菌门;浮霉菌门;硝化螺旋菌门;厚壁菌门;绿弯菌门;疣微菌门;绿菌门;放线菌门;红藻门以及螺旋体门。内外锈层的优势菌均为变形细菌门,分别占文库序列的46%和53%,其中α-变形菌纲又为门中优势类群,但在内外锈层中还存在明显的系统发育学分歧。

关 键 词:碳钢锈层  细菌多样性  16S  rDNA  Q235碳钢  微生物腐蚀

Diversity of Bacterial Community on the Surface of Q235 Steel in Temperate Coastal Marine Waters
LUAN Xin,DUAN Ji-Zhou,CHI Zhen-Ming.Diversity of Bacterial Community on the Surface of Q235 Steel in Temperate Coastal Marine Waters[J].Periodical of Ocean University of China,2012(Z1):107-115.
Authors:LUAN Xin  DUAN Ji-Zhou  CHI Zhen-Ming
Institution:1(1.College of Marine Life Sciences,Ocean University of China,Qingdao 266003,China;2.Institute of Oceanology,Chinese Academy of Sciences,Qingdao 266071,China)
Abstract:The bacterial diversity of the rust layer on Q235 steel immersed in seawater is studied.The bacterial community structures are investigated through 16S rDNA gene analysis,which results in 192 clones randomly selecting and sequencing.After the sequences are checked,64 OTUs are obtained in all,which indicates that the rust layer contain high bacterial diversity.These 64 clones could be divided into 13 phylotypes: Proteobacteria,Bacteroidetes,Bacillariophyta,Acidobacteria,Planctomycetes,Nitrospirae,Firmicutes,Chloroflexi,Verrucomicrobia,Chlorobi,Actinobacteria,Rhodophyta and Spirochaetes,but most of these sequences belong to uncultivated bacteria.Proteobacteria,especially Alphaproteobacteria,whose percentage is 46% and 53% respectively in the outer and inner rust layers,is the dominant component.The bacterial communities in the outer and inner rust layers also differ from each other in phylogenetical divergence.
Keywords:rust layer on steel  bacterial diversity  16S rDNA gene analysis  Q235 steel  microbial corrosion
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