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| 海洋硫酸多糖916在2种酶作用下稳定性的研究 | |
| 引用本文： | 王妍婷,吕志华,王远红.海洋硫酸多糖916在2种酶作用下稳定性的研究[J].中国海洋大学学报(自然科学版),2004,34(6):955-958,963. |
| 作者姓名： | 王妍婷 吕志华 王远红 |
| 作者单位： | 1. 中国海洋大学,海洋药物教育部重点实验室,山东,青岛,266003 2. 中国海洋大学,山东省海洋药物重点实验室,山东,青岛,266003 3. 中国海洋大学,海洋药物与食品研究所,山东,青岛,266003 |
| 基金项目： | 国家高技术发展计划项目 (2 0 0 2AA62 4 0 2 0 )资助 |
| 摘 要： | 利用高效凝胶渗透色谱法(HPGPC)和明胶比浊法研究了海洋硫酸多糖916在溶菌酶和胰蛋白酶作用下的稳定性，分别考察了酶作用下916的重均分子量、分子量分布系数和游离硫酸根含量的变化。结果表明：在所选定的实验条件下，916的重均分子量、分子量分布系数和游离硫酸根含量均未发生显著变化，表明在所选择的实验条件下916对溶菌酶和胰蛋白酶是稳定的。 |
| 关 键 词： | 海洋硫酸多糖916 酶 稳定性 |
| 文章编号： | 1672-5174(2004)06-955-04 |
Study on the Stability of Marine Sulfated Polysaccharide 916 by Two Enzymes |
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| WANG Yan-ting ,LV Zhi-hua ,WANG Yuan-hong.Study on the Stability of Marine Sulfated Polysaccharide 916 by Two Enzymes[J].Periodical of Ocean University of China,2004,34(6):955-958,963. | |
| Authors: | WANG Yan-ting LV Zhi-hua WANG Yuan-hong |
| Institution: | WANG Yan-ting 1,LV Zhi-hua 2,WANG Yuan-hong 3 |
| Abstract: | Two methods, including high performance gel permeation chromatography and gelatin nephelometry,are used to investigate the stability of marine sulfated polysaccharide 916 by lysozyme and trypsin, and weight average molecular, molecular weight distribution and dissociative sulfate content of 916 are determined. The results show that weight average molecular, molecular weight distribution and the dissociative sulfate content of 916 do not change obviously. It shows that 916 is stable by lysozyme and trypsin under this experimental condition. |
| Keywords: | marine sulfated polysaccharide 916 enzyme stability |
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