| 藻际可培养细菌对雨生红球藻生长和虾青素积累的影响 |
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| 引用本文： | 李芸,刘发龙,王巧晗,刘岩,李景玉,宫庆礼.藻际可培养细菌对雨生红球藻生长和虾青素积累的影响[J].中国海洋大学学报(自然科学版),2020,50(7):44-55. |
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| 作者姓名： | 李芸 刘发龙 王巧晗 刘岩 李景玉 宫庆礼 |
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| 作者单位： | 海水养殖教育部重点实验室(中国海洋大学) ,山东 青岛 266003;海水养殖教育部重点实验室(中国海洋大学) ,山东 青岛 266003;中国海洋大学水产学院,山东 青岛 266003 |
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| 基金项目： | 青岛海洋经济发展示范城市项目 |
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| 摘 要： | 为探究雨生红球藻(Haematococcus pluvialis)藻际可培养细菌多样性及可培养细菌对雨生红球藻生长和虾青素积累的影响,本实验采用培养分离法,在乙酸钠兼养条件下,共分离到8株雨生红球藻藻际细菌,通过16S rDNA测序鉴定,并在NCBI网站比较序列发现,编号为DBQ、HS、BTH、DBB、XBL、HB、FH、JH的菌株分别与Pseudomonas sp.、Methylobacterium sp.、Chryseobacterium sp.、Bacillus sp.、Paracoccus sp.、Planctopirus hydrillae、Rhodobacter vinaykumarii、Brevundimonas vesicularis的16S rDNA序列具有最高的相似性。分别将8株细菌与通过抗生素处理获得的纯种雨生红球藻共培养,测定了8株藻际细菌对雨生红球藻细胞密度、藻液pH、叶绿素及虾青素含量的影响。实验发现,HS处理组在绿色阶段培养第9天获得最高细胞密度,为3.65×105cells/mL,且显著高于对照组;DBB处理组虽然能够促进藻细胞密度增加,但与对照组并无显著差异;DBQ处理组藻细胞密度显著低于对照组及其他处理组。第1~7天,藻液叶绿素浓度的变化与藻细胞密度变化趋势有一定相似性,绿色阶段后期,叶绿素浓度呈现下降趋势,也说明藻细胞内部叶绿素向其他色素转变,同时标志着绿色阶段的结束。绿色阶段培养过程中,藻液pH变化幅度较大,由初始值7.0左右上升到9.30左右,但是培养后期处理组与对照组藻液pH并无显著差异。在测定藻液虾青素含量时,仅HS处理组虾青素含量略高于对照组,但并无显著差异;通过计算单个藻细胞虾青素含量发现DBQ处理组单个藻细胞虾青素含量显著高于对照组。
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| 关 键 词： | 混合营养培养 雨生红球藻 藻际细菌 生长 虾青素 |
Effects of Cultivable Phycosphere Bacteria on Growth of Haematococcus pluvialis and Its Astaxanthin Accumulation |
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| LI Yun,LIU Fa-Long,WANG Qiao-Han,LIU Yan,LI Jing-Yu,GONG Qing-Li.Effects of Cultivable Phycosphere Bacteria on Growth of Haematococcus pluvialis and Its Astaxanthin Accumulation[J].Periodical of Ocean University of China,2020,50(7):44-55. |
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| Authors: | LI Yun LIU Fa-Long WANG Qiao-Han LIU Yan LI Jing-Yu GONG Qing-Li |
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| Institution: | (The Key Laboratory of Mariculture (Ocean University of China), Ministry of Education, Qingdao 266003, China;College of Fisheries, Ocean University of China, Qingdao 266003, China) |
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| Abstract: | In order to explore the diversity of cultivable bacteria in Haematococcus pluvialis phycosphere and their effects on the growth of H.pluvialis and its astaxanthin accumulation,we used the traditional culture separation method to isolate 8 bacterial strains in the mixotrophic culture of H.pluvialis.Based on 16S rRNA gene sequences,strains(DBQ,HS,BTH,DBB,XBL,HB,FH and JH)were found to be highly similar with Pseudomonas sp.,Methylobacterium sp.,Chryseobacterium sp.,Bacillus sp.,Paracoccus sp.,Planctopirus hydrillae,Rhodobacter vinaykumarii and Brevundimonas vesicularis,respectively.We used 8 strains as the additives,and studied their effects on cell density,algal pH,and chlorophyll and astaxanthin contents of H.pluvialis purified ahead with antibiotics.We found that the HS obtained the highest cell density on the 9th day,which was 3.65×105 cells/mL,significantly higher than that of control.DBB increased the cell density;however no significant difference was found from that of control.From day 1 to day 7,the change in chlorophyll concentration was the same as that in algal cell density.At the end of green phase,the concentration of chlorophyll showed a decreasing trend,indicating that the chlorophyll in algal cells changed to other pigments and marked the end of green phase.At the green stage,the pH of algal liquid changed greatly,from the initial of 7.0 to about 9.30.However,there was no significant difference from that of control at the end of culture.The astaxanthin content of HS was slightly higher than that of control;however,no significant difference from that of control.It was also found that the content of astaxanthin in single algal cells of DBQ was significantly higher than that of control. |
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| Keywords: | mixotrophic culture Haematococcus pluvialis phycosphere bacteria growth astaxanthin |
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