| TYLCV-CHI侵染诱导的抗病毒 GFP标记的载体构建 |
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| 摘 要： | 以CTAB法提取含有番茄黄化曲叶病毒TYLCV-CHI病原的烟草曲 叶病烟草DNA为模板，扩增该病毒外壳蛋白启动子序列，克隆到pGEM7Z载体上，在启动子下 游连接上能表达毒素蛋白的RNase基因和终止子NOS，最终将构建的基因转移到带有绿色荧光 蛋白GFP的植物表达载体上，构建了通过病毒侵染后激发病毒外壳蛋白启动子从而表达毒素 基因，产生毒素杀死病毒防止感染继续扩散的自我保护机制的基因工程植物表达载体。
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| 关 键 词： | 中国番茄黄化曲叶病毒(TYLCV-CHI) 绿色莹光蛋白 外壳蛋 白启动子 毒素基因 CTAB法 C TAB method 陈刚 男 1972年9月出生 硕士。 |
| 修稿时间： | 2000-01-10 |
Construction GFP Marked Anti-virus Gene Infected and Induced by TYLCV-CHI |
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| Authors: | Chen Gang |
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| Abstract: | The total DNA is extracted by CATB from tobacco leaves of Tobacco Leaf Curl Disease which was inducted by TYLCV-CHI, amplify the viru s' coat protein promoter and clone it on Pgem 72 vector. Then connect the RN ase gene which can express toxin protein and terminator NOS on the promoter,at l ast translate the constucted gene on plant express vector marked with GFP.The pr omoter will express toxin gene when virus infected tobacco,then it can generate toxin to kill virus to prevent the infection from diffusion. |
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| Keywords: | TYLCV-CHI GFP coat protein promotor toxin gene |
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