| 增加贝类染色体分裂相的方法初探 |
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| 引用本文： | 贾志良,李智盈,包振民,潘洁,王如才.增加贝类染色体分裂相的方法初探[J].中国海洋大学学报(自然科学版),2001,31(2):232-236. |
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| 作者姓名： | 贾志良 李智盈 包振民 潘洁 王如才 |
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| 作者单位： | 青岛海洋大学, |
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| 基金项目： | 国家海洋“863”计划课题! (819- 0 4- 0 7)，国家重点基础研究发展规划项目课题! (G19990 12 0 0 9) |
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| 摘 要： | 研究以太平洋牡蛎为材料 ,用低温同步化法和 8μg/m L的植物血球凝集素 (PHA- M)、50μg/m L 伴刀豆蛋白 A(Con A)两种外源凝集素分别处理其鳃 ,后用鳃做染色体滴片 ,10 % Giemsa染色后镜检 ,统计分裂相数。发现三种方法都能使分裂相比例增加 ,其中低温同步化法效果最好 ,可使分裂相比例由对照的 0 .7× 10 -4 升高到 6 .5× 10 -4 ,提高 8.3倍。PHA- M法使分裂相比例由对照的 0 .7× 10 -4升高到 5.5× 10 -4 ,提高 6 .8倍 ,Con A法使分裂相比例由对照的 0 .7× 10 -4升高到3.6× 10 -4 ,提高 4 .1倍。
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| 关 键 词： | 太平洋牡蛎 分裂相比例 低温同步化法 外源凝集素 |
| 文章编号： | 1001-1862(2001)0 2-232-05 |
| 修稿时间： | 1999年9月20日 |
On Methods of Increasing the Metaphase of Shellfish |
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| Jia Zhiliang,Li Zhiying,Bao Zhenmin,Pan Jie,Wang Rucai.On Methods of Increasing the Metaphase of Shellfish[J].Periodical of Ocean University of China,2001,31(2):232-236. |
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| Authors: | Jia Zhiliang Li Zhiying Bao Zhenmin Pan Jie Wang Rucai |
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| Abstract: | Three methods, low temperature synchronizing and two lectins--8μg/mL PHA-M and 50μg/mL ConA, were used to treat the gill of Pacif ic oyster (Crassostrea gigas). The gill was dropped on a slide, stained with 10% Giemsa and then examined with microscope for the metaphase to be counted. T he proportion of metaphase increased after the gill was treated by all the three methods. We find that low temperature synchronizing is the best one. It can inc rease the proportion from 0.7×10-4 to 6.5×10-4, which means an increase of 8.3 times. PHA-M treatment can increase the proportion from 0. 7×10-4 to 5.5×10-4, the increase is 7.8 times, and ConA treatment can increase the proportion from 0.7×10-4 to 3.6×10-4, which is 4. 1 times. |
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| Keywords: | Pacific oyster proportion of metaphase low temperature synchronizing lectin |
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