| 赤潮异弯藻和海洋原甲藻LSU Rdna扩增及序列分析 |
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| 引用本文： | 董云伟,董双林.赤潮异弯藻和海洋原甲藻LSU Rdna扩增及序列分析[J].中国海洋大学学报(自然科学版),2004,34(3):358-364. |
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| 作者姓名： | 董云伟 董双林 |
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| 作者单位： | 中国海洋大学海水养殖教育部重点实验室,山东,青岛,266003 |
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| 基金项目： | 国家重点基础研究发展计划(973计划) |
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| 摘 要： | 利用引物D1R和D2C扩增并测定了赤潮异弯藻(Heterosigma akashiwo Hada)和海洋原甲藻(Prorocentrum micans Ehrenberg)的LSU rDNA D1与D2序列,并与GenBank中相关序列进行比较分析.结果表明:在种内水平,所测的H.akashiwo 6个株系之间共有5个变异位点,序列H.k与H.k-2,H.k-4均存在碱基替换;原甲藻属不同种内各株系之间的遗传距离为0.002～0.023之间,所测序列P.mi与P.micans其他株系之间均存在碱基替换.在种间水平上,原甲藻属不同种之间的遗传距离在0.045～0.139之间,大于种内遗传距离,每个种都具有特定的保守序列.根据序列间的遗传变异,可设计特异性的探针对不同株系和物种进行检测和计数.
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| 关 键 词： | 海洋原甲藻 赤潮异弯藻 特异性探针 |
| 文章编号： | 1672-1574(2004)03-358-07 |
| 修稿时间： | 2003年9月29日 |
Amplification and Analysis of Partial Ribosomal RNA Large Subnunit Genes Sequences of Heterosigma akashiwo Hada and Prorocentrum micans Ehrenberg |
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| DONG Yun-wei,DONG Shuang-lin.Amplification and Analysis of Partial Ribosomal RNA Large Subnunit Genes Sequences of Heterosigma akashiwo Hada and Prorocentrum micans Ehrenberg[J].Periodical of Ocean University of China,2004,34(3):358-364. |
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| Authors: | DONG Yun-wei DONG Shuang-lin |
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| Abstract: | The LSU rDNA partial sequences of marine algae, Heterosigma akashiwo Hada and Prorocentrum micans Ehrenberg were amplified with primers D1R/D2C and aligned with those in GenBank to assess the inter-and intraspecific variations. The intraspecific distances, calculated by the Kimura-2 parameters method, ranged form 0.002 to 0.023. Though the sequences were conservative within the same species, there were still some variation sites among different strains that could be used to design strains-specific probes. The genetic distances among species, within genus Prorocentrum, ranged from 0.045 to 0.139. Each species owns its conserved domains, by which species-specific probes can be designed to identify different Prorocentrum species. |
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| Keywords: | LSU rDNA |
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