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中国北方沿海6个栉孔扇贝群体的AFLP分析
引用本文:李宁,程洁,孙鲁阳,赵海波,侯睿,胡景杰,包振民.中国北方沿海6个栉孔扇贝群体的AFLP分析[J].中国海洋大学学报(自然科学版),2010,40(4).
作者姓名:李宁  程洁  孙鲁阳  赵海波  侯睿  胡景杰  包振民
作者单位:中国海洋大学海洋生物遗传育种教育部重点实验室,海洋生命学院,山东,青岛,266003
基金项目:国家高技术研究发展计划重大项目 
摘    要:利用4对AFLP引物首次对我国北方沿海主要的6个栉孔扇贝养殖区的自然采苗群体进行了遗传多样性分析,共扩增得到191条清晰带谱,其中175条具有多态性。对各群体进行遗传多样性分析,结果表明各群体均具有较高的遗传多样性水平,香农式多样性指数在0.347 4~0.391 7之间。AMOVA结果表明遗传变异主要来源于群体内,占总遗传变异的87.22%。个体聚类结果表明,烟台、长岛、大连3个群体的个体聚在一起,可看做1个群体;而荣成群体的个体单独聚在一起,具有独立的遗传结构;胶南和日照的一部分个体聚在一起,其他大部分各群体单独聚在一起;推测是海洋环境和人工养殖影响的共同结果。

关 键 词:栉孔扇贝(Chlamys  farreri)  自然苗群体  养殖区  遗传多样性

AFLP Analysis on Six Populations of Zhikong Scallop (Chlamys farreri) Along the Northern Coastline of China
LI Ning,CHENG Jie,SUN Lu-Yang,ZHAO Hai-Bo,HOU Rui,HU Jing-Jie,BAO Zhen-Min.AFLP Analysis on Six Populations of Zhikong Scallop (Chlamys farreri) Along the Northern Coastline of China[J].Periodical of Ocean University of China,2010,40(4).
Authors:LI Ning  CHENG Jie  SUN Lu-Yang  ZHAO Hai-Bo  HOU Rui  HU Jing-Jie  BAO Zhen-Min
Institution:LI+Ning,CHENG+Jie,SUN+Lu-Yang,ZHAO+Hai-Bo,HOU+Rui,HU+Jing-Jie,BAO+Zhen-Min(Key+Laboratory+of+Marine+Genetics+,+Breeding,Ministry+of+Education,College+of+Marine+Life,Ocean+University+of+China,Qingdao+266003,China)
Abstract:The AFLP technique was used to analyze the genetic diversity of 6 natural populations of Zhikong scallop(Chlamys farreri) from the main cultured zones along the northern coastline of China.Based on 4 pairs of primers,191 clear and repeated DNA fragments were generated,of which 175 were polymorphic.The genetic diversity analysis of the populations showed high levels of genetic diversity of 6 populations,and the Shannon s genetic diversity index varied from 0.347 4 to 0.391 7.The AMOVA(an-alyzing molecular va...
Keywords:AFLP  Zhikong scallop(Chlamys farreri)  natural seedlings population  cultured zone  AFLP  genetic diversity
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