| 海水循环水系统中环境因子对大西洋鲑体内土腥味物质含量的影响 |
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| 引用本文： | 魏发奕,韩萃,李丽,董双林,高勤峰.海水循环水系统中环境因子对大西洋鲑体内土腥味物质含量的影响[J].中国海洋大学学报(自然科学版),2021(3). |
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| 作者姓名： | 魏发奕 韩萃 李丽 董双林 高勤峰 |
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| 作者单位： | 海水养殖教育部重点实验（中国海洋大学）;青岛海洋科学与技术试点国家实验室 |
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| 基金项目： | 中国海洋大学与奥本大学联合研究中心项目;国家重点研究发展计划项目(2019YFD0901000)资助。 |
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| 摘 要： | 为获知不同时期封闭式海水循环水养殖系统中水质因子、浮游植物和细菌群落结构对大西洋鲑(Salmo salar)体内土腥味物质含量变化的影响,于2018—2019年4次采集封闭式海水循环水养殖系统的水样和大西洋鲑。测定水体的氨氮、亚硝氮、硝氮、活性磷酸盐等指标,分析水体浮游植物群落结构并通过16S高通量测序测定水体中细菌群落结构,通过微波蒸馏-固相微萃取-气相色谱-质谱联用(MD-SPME-GC-MS)方法,分析大西洋鲑背肌中土臭素(Geosmin,GSM)和二甲基异冰片(2-methylisoborneol,MIB)的含量。研究发现:测定水质指标中温度、溶解氧、盐度、pH、氨氮、亚硝氮、硝氮、活性磷酸盐、总氮、总磷、总悬浮物和溶解性总有机碳在不同时期差异显著。共鉴定出浮游植物7门、13目、26属,蓝藻门(Cyanophyta)、硅藻门(Bacillariophyta)和绿藻门(Chlorophyta)为优势门;不同时期细菌群落中的优势菌门均为变形菌门(Proteobacteria)和拟杆菌门(Bacteroidetes),但细菌群落结构存在差异,这主要是由水质差异引起的。在不同时期,鱼体MIB含量均高于GSM含量,MIB为鱼体中主要的土腥味物质,在7和10月MIB含量较高。鱼体土腥味物质含量受浮游植物和细菌群落影响,而水质因子可能通过影响浮游植物和细菌群落的结构,来影响养殖鱼体内土腥味物质的含量。
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| 关 键 词： | 大西洋鲑 循环水养殖系统 土臭素 二甲基异冰片 细菌群落 水质 |
Effects of Environmental Factors in Seawater Recirculating Aquaculture System on Concentrations of Off-Flavor Materials in Atlantic Salmon(Salmo salar) |
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| WEI Fa-Yi,HAN Cui,LI Li,DONG Shuang-Lin,GAO Qin-Feng.Effects of Environmental Factors in Seawater Recirculating Aquaculture System on Concentrations of Off-Flavor Materials in Atlantic Salmon(Salmo salar)[J].Periodical of Ocean University of China,2021(3). |
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| Authors: | WEI Fa-Yi HAN Cui LI Li DONG Shuang-Lin GAO Qin-Feng |
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| Institution: | (Key Laboratory of Mariculture (Ocean University of China), Ministry of Education, Qingdao 266003, China;Function Laboratory for Marine Fisheries Science and Food Production Processes, Pilot National Laboratory for Marine Science and Technology(Qingdao), Qingdao 266237, China) |
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| Abstract: | To understand the influences of water quality,phytoplankton communities and bacterial communities on the happening of off-flavor materials in Atlantic salmon(Salmo salar),water samples and Atlantic salmon were collected in different periods(April,July,October 2018 and January 2019)from a closed seawater recirculating aquaculture system.Water quality indexes such as total ammonia nitrogen and nitrate were measured,phytoplankton community structure in the culture water was analyzed and bacterial community structure was determined through 16s ribosomal RNA gene high-throughput sequencing.Geosmin(GSM)and 2-methylisoborneol(MIB)in Atlantic salmon were determined using microwave distillation with concomitant solid-phase microextraction coupled with gas chromatography-mass spectrum(MD-SPME-GC-MS).Results showed that the temperature,dissolved oxygen,salinity,pH,total ammonia nitrogen,nitrite nitrogen,nitrate nitrogen,soluble reactive phosphorus,total nitrogen,total phosphorus,total suspended solids and dissolved organic carbon were significantly different in different periods.There were 7 phyla,13 orders and 26 genera of phytoplankton identified from the system,and Cyanophyta,Bacillariophyta and Chlorophyta were the dominant phyla.The dominant phyla of bacterial communities in different periods were all Proteobacteria and Bacteroidetes,but there was difference in bacterial community structure among different periods,and this was mainly caused by difference in water quality.The concentration of MIB in fish was higher than that of GSM in different periods,and it was the main compound causing off-flavor in fish.The concentration of MIB was higher in July and October than the other two sampling times.The concentrations of MIB and GSM in fish were affected by phytoplankton and bacterial communities,moreover water quality indexes might affect the concentrations of off-flavor materials in cultured fish by affecting the structure of phytoplankton and bacterial communities. |
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| Keywords: | Salmo salar recirculating aquaculture system geosmin 2-methylisoborneol bacterial community water quality |
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