| DNA条形码技术及其在海洋贝类种质资源保护中的应用 |
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| 引用本文： | 李琪,冉轲,孔令锋.DNA条形码技术及其在海洋贝类种质资源保护中的应用[J].中国海洋大学学报(自然科学版),2021(5). |
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| 作者姓名： | 李琪 冉轲 孔令锋 |
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| 作者单位： | 海水养殖教育部重点实验室（中国海洋大学）;青岛海洋科学与技术试点国家实验室海洋渔业科学与食物产出过程功能实验室 |
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| 基金项目： | 中央高校基本科研业务费专项(201762014);国家自然科学基金项目(31772414)资助。 |
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| 摘 要： | DNA条形码技术是一种新兴的分子鉴定方法,是指通过选择一段DNA基因序列片段作为条形码进行物种鉴定、系统发育研究和物种多样性保护等。海洋贝类种类丰富、数量众多,传统的形态分类方法有一定的局限性,对一些非常见及疑难物种不能快速、准确地鉴定。作为一种快速、高效的物种诊断技术,DNA条形码技术在海洋贝类种质资源保护与利用方面起到了至关重要的作用。本文就DNA条形码的产生、原理、分析步骤、优势、局限及在海洋贝类种质资源保护中的应用进展进行了综述,并对其未来的发展进行了分析展望。
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| 关 键 词： | DNA条形码 海洋贝类 种质资源 物种诊断 |
DNA Barcoding and Its Application in Marine Shellfish Germplasm Resources Conservation |
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| LI Qi,RAN Ke,KONG Ling-Feng.DNA Barcoding and Its Application in Marine Shellfish Germplasm Resources Conservation[J].Periodical of Ocean University of China,2021(5). |
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| Authors: | LI Qi RAN Ke KONG Ling-Feng |
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| Institution: | (Key Laboratory of Mariculture(Ocean University of China), Ministry of Education, Qingdao 266003, China;Laboratory for Marine Fisheries Science and Food Production Processes, Pilot National Laboratory for Marine Science and Technology(Qingdao), Qingdao 266237, China) |
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| Abstract: | DNA barcoding is a modern molecular technique that can be used to identify species accurately.It has been widely used in species identification,phylogenetic research and biodiversity conservation.Marine shellfish is the most abundant groups of mollusks.It is essential to conduct a rapid and accurate identification of marine shellfish.As a rapid and efficient species identification technique,DNA barco-ding plays a key role in the conservation and utilization of marine shellfish germplasm resources.In this article,we introduced the generation,principle,workflow,advantages of DNA barcoding and reviewed its application in marine shellfish germplasm conservation and future prospects. |
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| Keywords: | DNA barcoding marine shellfish germplasm resource species identification |
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