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单体“海大1号”长牡蛎苗种室内人工培育模式的优化
引用本文:张哲,于瑞海,李海昆,马培振,李玲蔚,王永旺.单体“海大1号”长牡蛎苗种室内人工培育模式的优化[J].中国海洋大学学报(自然科学版),2021(2).
作者姓名:张哲  于瑞海  李海昆  马培振  李玲蔚  王永旺
作者单位:海水养殖教育部重点实验室(中国海洋大学);青岛国信蓝色硅谷发展有限责任公司
基金项目:山东省重点研究发展项目(2017GHY15124);海水养殖教育部重点实验室(中国海洋大学)开放基金项目(KLM2017004)资助。
摘    要:为提高单体“海大1号”长牡蛎(Crassostrea gigas)的培育效率,本研究以3种培育模式(下降流、上升流和静水)、6个水流量梯度(20、35、50、65、80和95 mL/s)和6个培育密度梯度(2.5、5、7.5、10、12.5和15个/mL)对单体“海大1号”长牡蛎苗种中间培育生长情况进行了研究。研究表明,单体“海大1号”长牡蛎苗种中间培育时,上升流和下降流均比静水培育效果好,培育前5天采用下降流、第6~29天采用上升流时,稚贝生长快、死亡率低;水流量小于65 mL/s时,随水流量的增加,稚贝生长加快,死亡率降低,水流量在65~80 mL/s时,单体牡蛎稚贝生长存活最优,第29天时最大壳高达(1534.81±65.23)μm。单体“海大1号”长牡蛎稚贝苗种中间培育时,随着培育密度的增大,挤迫效应逐渐显现,密度为7.5个/mL时,死亡率相对较低,且稚贝生长速度快,在第29天时稚贝壳高最大可达(1520.63±51.72)μm。基于以上研究结果,建议在规模化单体“海大1号”长牡蛎稚贝中间培育时,采用上升流和下降流相结合的流水培育方式,适当增加水流量,及时疏散密度。本研究优化了单体“海大1号”长牡蛎室内培育模式,为单体牡蛎规模化培育提供了参考方法和理论依据。

关 键 词:单体牡蛎  “海大1号”长牡蛎  稚贝  中间培育  培育模式  流水培育  培育密度

A Study on the Optimization of Indoor Artificial Cultivation Mode of Cultchless Crassostrea gigas Haida No.1
ZHANG Zhe,YU Rui-Hai,LI Hai-Kun,MA Pei-Zhen,LI Ling-Wei,WANG Yong-Wang.A Study on the Optimization of Indoor Artificial Cultivation Mode of Cultchless Crassostrea gigas Haida No.1[J].Periodical of Ocean University of China,2021(2).
Authors:ZHANG Zhe  YU Rui-Hai  LI Hai-Kun  MA Pei-Zhen  LI Ling-Wei  WANG Yong-Wang
Institution:(The Key Laboratory of Mariculture(Ocean University of China) ,Ministry of Education, Qingdao 266003,China;Qingdao Conson Oceantec Valley Development Co., Ltd, Qingdao 266200, China)
Abstract:To improve the indoor breeding efficiency of cultchless Crassostrea gigas Haida No.1,this research was carried out with three kinds of cultivation modes(downwelling,upwelling,still water),six water flows(20,35,50,65,80 and 95 mL/s)and six breeding densities(7.5,2.5,5,10,12.5 and 15 inds/mL)of cultchless oyster among the growth.The results showed that the juveniles cultivated in upwelling and downwelling were better than those in still water in transitional cultivation.In the first five days of downwelling breeding and from days 6 to 29 day of upwelling breeding,the spat grew fast and the mortality rate decreased.When water flow was less than 65 mL/s,the growth rate of juveniles accelerated and their mortality rate declined as the amount of water flow increased.When water flow varied between 65 and 80 mL/s,the condition for cultchless oyster growth is the best and the shell height of juvenile reached the maximum of(1534.81±65.23)μm on 29th day.In the transitional cultivation of juveniles,the squeezing effect gradually appeared with the increase of the cultivation density.The mortality rate of cultchless oyster was relatively low when the density was 7.5 inds/mL,and the shell height of juvenile oyster reached the maximum of(1520.63±51.72)μm on 29th day.Based on the above results,in cultchless C.gigas“Haida No.1”transitional cultivation,it is suggested that the method of flowing water with the combination of upwelling and downwelling,properly increasing the water flow and evacuating the density in time should be adopted so as to ensure the bait and oxygen supply.This study optimized the indoor cultivation mode of cultchless C.gigas Haida No.1,and provided reference methods and theories for large-scale cultivation of cultchless oyster.
Keywords:cultchless oyster  Crassostrea gigas Haida No  1  spat  transitional cultivation  cultivation mode  running water cultivation  cultivation density
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