| 海绵内生真菌胞外多糖理化性质及自由基清除活性研究 |
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| 引用本文： | 陈荫,郭守东,徐健,陈艳丽,李红燕,齐晓辉,毛文君.海绵内生真菌胞外多糖理化性质及自由基清除活性研究[J].中国海洋大学学报(自然科学版),2010,40(5). |
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| 作者姓名： | 陈荫 郭守东 徐健 陈艳丽 李红燕 齐晓辉 毛文君 |
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| 作者单位： | 中国海洋大学医药学院,山东,青岛,266003 |
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| 摘 要： | 从1株海绵内生真菌(Alternaria sp.)链格孢菌的发酵液中提取胞外多糖,对其进行Q Sepharose FF离子交换色谱分离纯化,得到2个组分JJY-W和JJY-S。运用各种化学方法及波谱方法对JJY-W和JJY-S的理化性质及结构进行分析,并对多糖清除自由基活性进行了评价。结果表明,JJY-W和JJY-S的分子量分别为1.4KD和1.8KD;JJY-W以半乳糖、葡萄糖为主,含有少量甘露糖,摩尔比为:甘露糖∶葡萄糖∶半乳糖=1∶2.5∶11。JJY-S以甘露糖、葡萄糖为主,含有少量半乳糖,摩尔比为:甘露糖∶葡萄糖∶半乳糖=3∶2∶1。JJY-W总糖含量为46.3%,未检测出糖醛酸,蛋白含量为2%。JJY-S总糖含量为52%,糖醛酸含量为6.1%,蛋白含量为14%。活性分析表明,2种多糖均具有一定的体外抗氧化活性,并随着浓度的增加清除自由基能力均增强,JJY-W清除DPPH自由基的活性要强于JJY-S,而JJY-S清除OH.自由基的活性要强于JJY-W。
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| 关 键 词： | 海绵真菌 胞外多糖 分离纯化 理化性质 自由基清除 |
Studies on Exopolysaccharides from a Marine Endogenetic Fungus of the Sponge |
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| CHEN Yin,GUO Shou-Dong,XU Jian,CHEN Yan-Li,LI Hong-Yan,QI Xiao-Hui,MAO Wen-Jun.Studies on Exopolysaccharides from a Marine Endogenetic Fungus of the Sponge[J].Periodical of Ocean University of China,2010,40(5). |
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| Authors: | CHEN Yin GUO Shou-Dong XU Jian CHEN Yan-Li LI Hong-Yan QI Xiao-Hui MAO Wen-Jun |
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| Institution: | CHEN Yin,GUO Shou-Dong,XU Jian,CHEN Yan-Li,LI Hong-Yan,QI Xiao-Hui,MAO Wen-Jun(School of Medicine and Pharmacy,Ocean University of China,Qingdao 266003,China) |
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| Abstract: | The crude exopolysaccharides were extracted from the fermented liquid of marine-derived endogenetic fungus of the sponge(Alternaria sp.),and further were purified by ion-exchange chromatography.Two polysaccharides JJY-W and JJY-S were obtained.The chemical properties of the two polysaccharides were investigated by various chemical and spectroscopic methods.The results showed that the molecular weights of JJY-W and JJY-S were 1.4KD and 1.8KD,respectively.JJY-W was mainly composed of galactose and glucose wit... |
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| Keywords: | marine fungus exopolysaccharides purification chemical properties antioxidant activity |
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