| 刺参中皂苷组分蒸发光散射-高效液相特征图谱研究 |
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| 引用本文： | 吕志华,马福刚,王远红,姜廷福.刺参中皂苷组分蒸发光散射-高效液相特征图谱研究[J].中国海洋大学学报(自然科学版),2010,40(3). |
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| 作者姓名： | 吕志华 马福刚 王远红 姜廷福 |
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| 作者单位： | 中国海洋大学医药学院,海洋药物教育部重点实验室,山东,青岛,266003 |
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| 基金项目： | 国家高技术研究发展计划项目 |
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| 摘 要： | 采用高效液相色谱-蒸发光散射(HPLC-ELSD)法建立了刺参皂苷的特征图谱.方法:色谱柱为Symmetry C18(150 mm×4.6 mm,5 μm),采用0.2% 乙酸水溶液-乙腈进行梯度洗脱,流速1.0 mL/min;柱温30 ℃;漂移管温度70 ℃;载气压力25 psi.结论:方法具有良好的专属性,可以作为刺参类产品是否含有刺参皂苷的评价方法.
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| 关 键 词： | 刺参 皂苷 特征图谱 |
Study on the Characteristic Chromatogram of Glycosides of Apostichopus japonicus by HPLC-ELSD |
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| LV Zhi-Hua,MA Fu-Gang,WANG Yuan-Hong,JIANG Ting-Fu.Study on the Characteristic Chromatogram of Glycosides of Apostichopus japonicus by HPLC-ELSD[J].Periodical of Ocean University of China,2010,40(3). |
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| Authors: | LV Zhi-Hua MA Fu-Gang WANG Yuan-Hong JIANG Ting-Fu |
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| Institution: | LV+Zhi-Hua,MA+Fu-Gang,WANG+Yuan-Hong,JIANG+Ting-Fu(Key+Laboratory+of+Marine+Drugs,Ministry+of+Education,School+of+Medicine+,+Pharmacy,Ocean+University+of+China,Qingdao+266003,China) |
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| Abstract: | A HPLC-ELSD Characteristic chromatogram of glycosides extracted from Apostichopus japonicus using HPLC-ELSD was established. The method used a Waters Symmetry C18 column. The mobile phase consisted of 0.2% HAc water solution and acetonitrile using a gradient elution. The flow rate was 1.0 mL/min and the column temperature was set at 30 ℃. The drift tube temperature of ELSD was set at 70 ℃ with a carrier air pressure of 25 psi. Conclusion: The specificity of this method to glycosides of Apostichopus japonicus is good. The method can be used as an assessment tool of glycosides of Apostichopus japonicus. |
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| Keywords: | HPLC-ELSD Apostichopus japonicus HPLC-ELSD glycosides characteristic chromatogram |
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