| 海萝藻(Gloiopeltis furcata)多糖的提取分离及其结构表征 |
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| 引用本文： | 于广利,胡艳南,杨波,赵峡,王培培,嵇国利,吴建东,管华诗.海萝藻(Gloiopeltis furcata)多糖的提取分离及其结构表征[J].中国海洋大学学报(自然科学版),2009,39(5). |
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| 作者姓名： | 于广利 胡艳南 杨波 赵峡 王培培 嵇国利 吴建东 管华诗 |
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| 作者单位： | 中国海洋大学医药学院糖科学与糖工程实验室,山东,青岛,266003 |
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| 基金项目： | 科技部国际合作项目(2007DFA30980);;国家海洋高技术研究发展计划项目(2007AA09Z445)资助 |
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| 摘 要： | 以海萝藻(Gloiopeltis furcata)为原料,依次经冷水、热水和热碱溶液提取,从中获得了3种多糖(GFW,GFH和GFA),其得率分别为57.9%,2.5%和2.6%。运用气相色谱法(GC)、高效凝胶渗透色谱法(HPGPC)、红外光谱(IR)及核磁共振碳谱(13C-NMR)等方法对其单糖组成、分子量以及结构进行了分析。结果表明,GFW和GFH都只含有D-半乳糖(Gal)和3,6-内醚-L-半乳糖(AnG),而GFA除了含有Gal和AnG外,还有木糖;3种多糖的分子量分别为22.6 kD,26.5 kD和49.8 kD;硫酸基含量分别为31.2%,25.1%,22.7%。IR和13C-NMR数据均表明其硫酸基在半乳糖残基C6位。结论:海萝藻中多糖均为硫酸多糖,其中2种为硫琼胶,另一种为含有木糖的硫酸半乳聚糖。
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| 关 键 词： | 海萝藻 硫酸半乳聚糖 提取 结构表征 |
Extraction, Isolation and Structural Characterization of Polysaccharides from a Red Alga Gloiopeltis furcata |
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| YU Guang-Li,HU Yan-Nan,YANG Bo,ZHAO Xia,WANG Pei-Pei,JI Guo-Li,WU Jian-Dong,GUAN Hua-Shi.Extraction, Isolation and Structural Characterization of Polysaccharides from a Red Alga Gloiopeltis furcata[J].Periodical of Ocean University of China,2009,39(5). |
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| Authors: | YU Guang-Li HU Yan-Nan YANG Bo ZHAO Xia WANG Pei-Pei JI Guo-Li WU Jian-Dong GUAN Hua-Shi |
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| Institution: | Laboratory of Glycochemistry and Glycoengineering;School of Medicine and Pharmacy;Ocean University of China;Qingdao 266003;China |
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| Abstract: | Three kinds of polysaccharides GFW,GFH and GFA were extracted from a red alga Gloiopeltis furcata by cold water,hot water and hot alkaline solution with the yield of 57.9%,2.5% and 2.6%,respectively.Their monosaccharide composition,weight averaged molecular mass and structural characters were determined by gas chromatography(GC),high performance gel permeation chromatography(HPGPC),Fourier transform infrared spectroscopy(FTIR) and 13C-NMR spectroscopy,separately.The experimental results showed that GFW and ... |
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| Keywords: | Gloiopeltis furcata sulfated galactan extraction structural characterization |
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