| 大菱鲆亲子鉴定的微卫星多重PCR技术建立及应用 |
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| 引用本文： | 苗贵东,杜民,杨景峰,徐营,樊廷俊,陈松林.大菱鲆亲子鉴定的微卫星多重PCR技术建立及应用[J].中国海洋大学学报(自然科学版),2011(Z1):97-106. |
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| 作者姓名： | 苗贵东 杜民 杨景峰 徐营 樊廷俊 陈松林 |
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| 作者单位： | 中国海洋大学海洋生命学院;中国水产科学研究院黄海水产研究所;上海海洋大学水产与生命学院; |
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| 基金项目： | 国家自然科学基金(30671623); 山东省泰山学者工程专项资助 |
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| 摘 要： | 用8个微卫星标记组合建立了2个微卫星多重PCR体系,对大菱鲆7个人工选育家系进行了系谱认证、亲子鉴定和遗传多样性研究。2个多重PCR体系中8个微卫星位点共检测到等位基因49个,每个位点等位基因数为3~8个。根据已知亲本及子代基因型,成功推断出了3个缺失亲本的基因型。在双亲未知的情况下2个多重PCR的8个微卫星位点累积排除概率是96.58%,已知1个亲本时累积排除率为99.71%,亲子鉴定准确率为96.42%。采用70个个体进行双盲验证,利用UPGMA法对7个家系的70个体进行了聚类分析,同一家系95.71%的个体聚类分析结果与系谱来源一致。Cervus 2.0软件亲子鉴定结果表明亲子鉴定准确率为92.86%。2个多重PCR体系的建立为大菱鲆不同家系混养后的亲子鉴定、系谱分析和分子辅助家系管理提供了技术手段。
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| 关 键 词： | 大菱鲆 微卫星 多重PCR 亲子鉴定 系谱认证 |
Development of Two Microsatellite Multiplex PCR Systems for Turbot(Scophthalmu smaximus) and Its Application for Family Management |
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| MIAO Gui-Dong,DU Min,YANG Jing-Feng,XU Ying,FAN Ting-Jun,CHEN Song-Lin.Development of Two Microsatellite Multiplex PCR Systems for Turbot(Scophthalmu smaximus) and Its Application for Family Management[J].Periodical of Ocean University of China,2011(Z1):97-106. |
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| Authors: | MIAO Gui-Dong DU Min YANG Jing-Feng XU Ying FAN Ting-Jun CHEN Song-Lin |
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| Institution: | MIAO Gui-Dong1,2,DU Min2,3,YANG Jing-Feng2,XU Ying3,FAN Ting-Jun1,CHEN Song-Lin2(1.College of Marine Life Sciences,Ocean University of China,Qingdao 266003,China,2.Key Lab for Sustainable Utilization of Marine Fisheries Resources,Ministry of Agriculture,Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Qingdao 266071,3.College of Aqua-life Science and technology,Shanghai Ocean University,Shanghai 200090,China) |
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| Abstract: | We developed two microsatellite multiplex PCR systems using 8 microsatellite loci for turbot(Scophthalmus maximus),which is one important aquaculture species in China.The multiplex PCR microsatellite assay was used to estimate the kinship and the genetic diversity of seven turbot families.Based on the genotypes of either known parent or none of them and offspring,all the absent parental genotypes of the seven families were deduced.According to the unique alleles among the families,each family could be disti... |
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| Keywords: | turbot(Scophthalmus maximus) microsatellite multiplex PCR parentage determination genealogical identification |
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