| 中华绒螯蟹与日本绒螯蟹线粒体COI基因片段的序列比较研究 |
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| 引用本文： | 孔晓瑜,喻子牛,刘亚军,高天翔,武云飞.中华绒螯蟹与日本绒螯蟹线粒体COI基因片段的序列比较研究[J].中国海洋大学学报(自然科学版),2001,31(6):861-866. |
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| 作者姓名： | 孔晓瑜 喻子牛 刘亚军 高天翔 武云飞 |
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| 作者单位： | 1. 青岛海洋大学水产学院,
2. 室青岛海洋大学水产学院, |
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| 基金项目： | 高等学校骨干教师资助计划项目，留学回国人员科研启动基金资助 |
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| 摘 要： | 以相应引物 PCR扩增了黄河口中华绒螯蟹线粒体细胞色素氧化酶 I亚基基因 (COI)片段 ,PCR产物经 T载体连接之后进行克隆、测序 ,得到 70 9bp的碱基序列 ,其 A,T,G,C含量分别为 34.4 1% ,2 7.93% ,2 0 .0 3%和 17.6 3%。并比较它与珠江流域中华绒螯蟹 COI序列和日本绒螯蟹 COI序列的差异 ,发现黄河口中华绒螯蟹与珠江流域中华绒螯蟹 COI序列完全相同 ,而与日本绒螯蟹差异非常明显 ,70 9或 6 5 8(不计引物 )位点中核苷酸差异数为 32 ,核苷酸差异率为 4 .5 1%或 4 .86 % (不计引物 ) ,其中 2 5个位点为转换 ,7个位点为颠换。作者倾向于支持存在中华绒螯蟹和日本绒螯蟹 ,或它们为同一种的两个地理亚种的观点
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| 关 键 词： | 中华绒螯蟹 日本绒螯蟹 细胞色素氧化酶I亚基基因(COI) 序列测定 |
| 文章编号： | 1001-1862(2001)06-861-06 |
| 修稿时间： | 2001年6月8日 |
Comparative Study of Mitochondrial COI Gene Fragment Between Chinese Mitten Crab (Eriocheir sinensis)and Japanese Mitten Crab (Eriocheir japonica) |
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| Kong Xiaoyu,Yu Ziniu,Liu Yajun,Gao Tianxiang,Wu Yunfei.Comparative Study of Mitochondrial COI Gene Fragment Between Chinese Mitten Crab (Eriocheir sinensis)and Japanese Mitten Crab (Eriocheir japonica)[J].Periodical of Ocean University of China,2001,31(6):861-866. |
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| Authors: | Kong Xiaoyu Yu Ziniu Liu Yajun Gao Tianxiang Wu Yunfei |
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| Abstract: | Mitochondrial COI gene fragment of Chinese mitten crab ( Eriocheir sinensis ) from Yellow River was amplified via PCR, the PCR products were ligated into T vector, cloned and sequenced. 709 bp nucleotide sequences were retrieved. The content of A, T, G and C in the fragment were 34.41%, 27.93%, 20.03% and 17.63% respectively. The sequence comparison was made among Chinese mitten crab from Yellow River and from Zhujiang River and Japanese mitten crab ( E. japonica ). Chinese mitten crabs from Yellow River and from Zhujiang River had the same nucleotide sequence, but they differed significantly from Japanese one; nucleotide difference number between the two species was 32, in which 25 were transitions and 7 were transversions. The result tended to support the opinion that Chinese mitten crab and Japanese mitten crab could be two species or different geographicsubspecies of the same species. |
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| Keywords: | Eriocheir sinensis Eriocheir japonica Mitochondrial COI gene sequencing |
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