| 条斑紫菜丝状孢子体cDNA文库构建及抗病相关基因鉴定 |
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| 引用本文： | 杨官品,刘永健,孙雪,沈怀舜,许璞,张学成.条斑紫菜丝状孢子体cDNA文库构建及抗病相关基因鉴定[J].中国海洋大学学报(自然科学版),2003,33(1):47-52. |
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| 作者姓名： | 杨官品 刘永健 孙雪 沈怀舜 许璞 张学成 |
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| 作者单位： | 1. 中国海洋大学海洋生命学院,青岛,266003
2. 江苏省海洋水产研究所,南通,226007 |
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| 基金项目： | 江苏省海洋水产研究所所长基金项目资助 |
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| 摘 要： | 经过微量总 RNA抽提、c DNA合成、c DNA扩增和克隆等步骤 ,构建了微量条斑紫菜丝状孢子体 c DNA文库 ,并进行了小规模表达序列标签分析。从 1 70条标签序列中鉴定出 6条抗病相关基因序列。这些序列可用于研制抗病单核苷酸多态性标记 ,辅助紫菜抗性遗传改良。
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| 关 键 词： | 条斑紫菜 cDNA PCR文库 cDNA文库 表达序列标签 抗病基因 |
| 文章编号： | 1001-1862(2003)01-047-06 |
| 修稿时间： | 2002年7月9日 |
Construction of the cDNA Library of Filamentous Laver Sporophyte and the Identification of Disease Resistance Gene Tags |
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| Yang Guanpin,Liu Yongjian,Sun Xue,Shen Huaishun,Xu Pu,Zhang Xuecheng.Construction of the cDNA Library of Filamentous Laver Sporophyte and the Identification of Disease Resistance Gene Tags[J].Periodical of Ocean University of China,2003,33(1):47-52. |
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| Authors: | Yang Guanpin Liu Yongjian Sun Xue Shen Huaishun Xu Pu Zhang Xuecheng |
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| Institution: | Yang Guanpin1 Liu Yongjian1 Sun Xue1 Shen Huaishun2 Xu Pu2 Zhang Xuecheng1 |
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| Abstract: | The cDNA library was constructed from a small amount of filamentous sporophyte of Porphyra yezoensis by the PCR amplification of cDNA. The protocol includes steps such as the extraction of total RNA, cDNA synthesis, cDNA amplification and cloning.The constructed cDNA library has been used for expressed sequence tag analysis, and six disease resistance gene tags were identified. These tags hold the potential for being developed into single nucleotide polymorphism markers, which will enhance the genetic improvement of laver resistance performance. |
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| Keywords: | Porphyra yezoensis cDNA PCR library cDNA library expressed sequence tag disease resistance gene |
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