| 三疣梭子蟹线粒体DNA 16S rRNA和COI基因片段序列的比较研究 |
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| 引用本文： | 郭天慧,孔晓瑜,陈四清,喻子牛.三疣梭子蟹线粒体DNA 16S rRNA和COI基因片段序列的比较研究[J].中国海洋大学学报(自然科学版),2004,34(1):22-28. |
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| 作者姓名： | 郭天慧 孔晓瑜 陈四清 喻子牛 |
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| 作者单位： | 1. 中国海洋大学教育部海水养殖重点实验室,山东,青岛,266003
2. 中国水产研究院黄海水产研究所,山东,青岛,266071 |
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| 基金项目： | 海洋渔业资源可持续利用农业部重点开放实验室资助 |
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| 摘 要： | 本文采用 PCR扩增和序列测定等技术 ,对三疣梭子蟹 (Portunustrituberculatus)线粒体DNA1 6Sr RNA和 COI基因片段进行了初步研究。经 PCR扩增和序列测定 ,分别得到 1 6Sr RNA和 COI2个基因片段的碱基序列 ,其中 1 6Sr RNA基因片段的大小为 566bp,碱基 A,T,G,C的含量分别为 35.1 6% ,34.45% ,1 2 .37%和 1 8.0 2 % ;COI基因片段的大小为 658bp,碱基 A,T,G,C的含量分别为 36.63% ,2 6.44% ,2 0 .52 %和 1 6.41 %。在 2种基因片段中 ,AT含量均明显高于 GC含量 ,这与果蝇、虾类、蟹类等无脊椎动物的 1 6Sr RNA和 COI基因片段研究结果相似。通过对三疣梭子蟹 1 6S r RNA和 COI2个基因片段遗传特征的研究 ,发现其种内变异较低 ,在 3个样本中 1 6Sr RNA基因片段序列完全一样 ,COI基因片段中也只有 2个 T/ C位点转换。另外 ,比较本研究所得序列与 Gen Bank中梭子蟹科 5个属的 1 6Sr RNA基因片段序列后 ,发现其聚类结果与传统分类相一致。
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| 关 键 词： | 三疣梭子蟹 16S rRNA基因 COI基因 PCR |
| 文章编号： | 1672-1574(2004)01-022-07 |
| 修稿时间： | 2002年12月10 |
A Study of the Sequences of Mitochondrial 16S rRNA and COI Gene Fragments of Portunus trituberculatus |
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| GUO Tian-hui ,KONG Xiao-yu ,CHEN Si-qing ,YU Zi-niu.A Study of the Sequences of Mitochondrial 16S rRNA and COI Gene Fragments of Portunus trituberculatus[J].Periodical of Ocean University of China,2004,34(1):22-28. |
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| Authors: | GUO Tian-hui KONG Xiao-yu CHEN Si-qing YU Zi-niu |
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| Institution: | GUO Tian-hui 1,KONG Xiao-yu 1,CHEN Si-qing 2,YU Zi-niu1 |
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| Abstract: | Mitochondrial 16S rRNA and COI gene fragments of Portunus trituberculatus from Weifang were amplified via PCR, then the PCR products were purified and sequenced. For 16S rRNA gene fragments, 566bp nucleotide sequences were obtained, and the A, T, G and C contents in this fragment were respectively 35.16%, 34.45%, 26.44% and 18.02%.As for the COI gene fragments, the size was 658bp and the A, T, G and C contents were 36.63%, 26.44%, 20.52% and 16.41% respectively. Analysis of 16S rRNA and COI gene fragments indicated that: 1) the AT content was higher than CG content, which was similar to the results of studies on drosophila, shrimp, crab and other invertebrates; 2) three samples from the test had the completely same nucleotide sequence of 16S rRNA gene fragments, but there were two locis of T/C translation about the sequence of COI gene fragments. In addition, it is found that the graphical phylogenetic tree of 16S gene fragment of eight orabs in five genus is consistent with current systematics of these species. |
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| Keywords: | Portunus trituberculatus 16S rRNA gene COI gene PCR |
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