| UV-B辐射对2种海洋微藻膜脂过氧化和脱酯化伤害 |
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| 引用本文： | 王军,肖慧,冯蕾,蔡恒江,唐学玺.UV-B辐射对2种海洋微藻膜脂过氧化和脱酯化伤害[J].中国海洋大学学报(自然科学版),2006,36(5):763-766. |
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| 作者姓名： | 王军 肖慧 冯蕾 蔡恒江 唐学玺 |
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| 作者单位： | 1. 中国海洋大学海洋生态实验室,山东,青岛,266003
2. 中国海洋大学海洋生态实验室,山东,青岛,266003;东营职业学院,山东,东营,257091 |
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| 基金项目： | 国家自然科学基金;国家新世纪优秀人才支持计划项目 |
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| 摘 要： | 研究海洋生态系统在调节全球变化中的作用,采用生态毒理学的方法研究了UV-B辐射增强对2种海洋微藻膜的损伤及其差异性。结果表明,随着UV-B辐射剂量的增加,叉鞭金藻(Dicrateriasp.)和三角褐指藻(Phaeodactylum tricornu-tum)的光合速率降低。同时,膜相对透性增大,细胞内H2O2含量上升,微粒体膜中磷脂减少,游离脂肪酸增加。表明UV-B辐射增强使2种微藻的膜受到了严重伤害,而且膜伤害的加剧与活性氧的积累和由此引发的膜脂脱酯化有关。2种微藻细胞匀浆中丙二醛(MDA)含量无显著变化,而微粒体膜的MDA含量随着UV-B辐射剂量的加大显著提高。这表明UV-B辐射增强对2种海洋微藻膜的损伤可能是由膜脂过氧化和脱酯化作用共同引起的。
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| 关 键 词： | UV-B辐射增强 膜脂过氧化 脱酯化 海洋微藻 |
| 文章编号： | 1672-5174(2006)05-763-04 |
| 收稿时间： | 2006-05-22 |
| 修稿时间： | 2006-06-21 |
Studies on Damage of Membrane Lipid Peroxidation and Deesterification Caused by UV-B Radiation in Two Species of Marine Microalgae |
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| WANG Jun,XIAO Hui,FENG Lei,CAI Heng-Jiang,TANG Xue-Xi.Studies on Damage of Membrane Lipid Peroxidation and Deesterification Caused by UV-B Radiation in Two Species of Marine Microalgae[J].Periodical of Ocean University of China,2006,36(5):763-766. |
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| Authors: | WANG Jun XIAO Hui FENG Lei CAI Heng-Jiang TANG Xue-Xi |
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| Institution: | 1. Marine Ecology Laboratory, Ocean University of China, Qingdao 266003, China; 2. Dong Ying Vocation College, Dongying 257091, China |
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| Abstract: | The membrane damage and its differences caused by UVB radiation enhancement to two species of marine microalgae were studied using ecotoxicology methods.The results showed that the membrane relative permeability and H_(2)O_(2) content increased,and the photosynthesis rate decreased in Dicrateria sp.and Phaeodactylum tricornutum with the UV-B radiation enhancement.Meanwhile,phospholipid content decreased and free fatty acid content promoted in microsomal membrane.These indicated that the membrane damage in the two species of marine microalgae was seriously induced by UV-B radiation enhancement,and the exacerbation of membrane damage was really relative to the accumulation of active oxygens which initiated deesterification.Although malondiadehyde(MDA) content in homogenate of two species of marine microalgae did not show significant change,it exhibited obvious increment in microsomal membrane.So it was concluded that the membrane damage under UV-B radiation enhancement was caused by membrane lipid peroxidation in combination with membrane lipid deesterification. |
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| Keywords: | UV-B radiation enhancement membrane lipid peroxidation deesterification marine microalgae |
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