| 生物固氮速率测定中15N2同位素示踪剂的引入 |
| |
| 引用本文： | 陈盛保,张润,陈能汪,赖丽旻,陈敏,郑敏芳,邱雨生.生物固氮速率测定中15N2同位素示踪剂的引入[J].海洋学报,2018,40(10):42-50. |
| |
| 作者姓名： | 陈盛保 张润 陈能汪 赖丽旻 陈敏 郑敏芳 邱雨生 |
| |
| 作者单位： | 1.厦门大学 海洋与地球学院, 福建 厦门 361102 |
| |
| 基金项目： | 国家重点基础研究发展计划（2015CB452903）；科技基础资源调查专项子课题（2017FY201403）；国家自然科学基金（41676174）。 |
| |
| 摘 要： | 生物固氮作用是一个重要的海洋新氮来源，在海洋生物地球化学循环中扮演着不可替代的角色。基于稳定同位素（15N2）示踪吸收法，是目前直接测定海洋生物固氮速率最有效的手段。其中，高效、洁净地将15N2引入海水培养体系，并准确定量培养体系底物的同位素示踪水平，是同位素示踪吸收法准确获取固氮速率的关键。本研究针对15N2同位素示踪剂引入这一关键环节进行了探讨，确认改进气泡法是将15N2引入海水培养体系的首选操作。在对培养体系造成的较小扰动的情况下，可将培养体系氮气底物的15N原子丰度提升至10%以上，相对于另一种导入同位素示踪剂的手段——预溶解海水法，改进气泡法将培养瓶中氮气底物的15N原子丰度提升了近200%。此外，改进气泡法还具有最小化痕量金属沾污、操作简便等优点。将改进气泡法结合与稳定同位素比值质谱测定结合，是准确测定水体生物固氮速率的推荐方法。
|
| 关 键 词： | 生物固氮速率 15N2 同位素示踪 |
| 收稿时间： | 2018/4/15 0:00:00 |
| 修稿时间： | 2018/6/29 0:00:00 |
On the introduction of 15N2 tracer in marine N2 fixation rate assay |
| |
| Chen Shengbao,Zhang Run,Chen Nengwang,Lai Limin,Chen Min,Zheng Minfang and Qiu Yusheng.On the introduction of 15N2 tracer in marine N2 fixation rate assay[J].Acta Oceanologica Sinica (in Chinese),2018,40(10):42-50. |
| |
| Authors: | Chen Shengbao Zhang Run Chen Nengwang Lai Limin Chen Min Zheng Minfang and Qiu Yusheng |
| |
| Institution: | 1.College of Ocean and Earth Sciences, Xiamen University, Xiamen 361102, China2.College of the Environment and Ecology, Xiamen University, Xiamen 361102, China |
| |
| Abstract: | N2 fixation plays an essential role in marine biogeochemical cycles by supplying new N. Stable isotope tracer (15N2) assay has been considered as the best approach for measuring in situ N2 fixation rate. Introducing 15N2 in an efficient and clean way, and precisely determining the isotopic enrichment level in the incubation system, is thus fundamental for obtaining true rates. In this study, we show that modified bubble treatment is a recommended practice in introducing 15N2. It can create 15N atomic abundance of substrate N2 in the incubation bottle higher than 10%. Such enrichment is twice higher when compared with gas pre-dissolution treatment. Moreover, modified bubble treatment is featured by much lowered risk of trace metal contamination and less time/labor consuming. We propose that the modified bubble method coupled to stable isotope mass spectrometry should be widely used in measuring N2 fixation rate in aquatic environments. |
| |
| Keywords: | N2 fixation rate 15N2 isotope tracer assay |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《海洋学报》浏览原始摘要信息 |
| 点击此处可从《海洋学报》下载免费的PDF全文 |
|
