| 对虾白斑杆状病毒基因中的小顺反子及其表达 |
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| 引用本文： | 章晓波,徐洵,杨丰.对虾白斑杆状病毒基因中的小顺反子及其表达[J].海洋学报,2001,23(1):73-78. |
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| 作者姓名： | 章晓波 徐洵 杨丰 |
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| 作者单位： | 1.中国科学技术大学生命科学学院, 安徽合肥 230026 |
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| 基金项目： | 国家“863”计划资助项目(219-02-05-01). |
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| 摘 要： | 通过对虾白斑杆状病毒DNA文库和cDNA文库的碱基序列测定和分析,发现对虾白斑杆状病毒的一个开放阅读框的前导序列中含有一个小顺反子,这个小顺反子较多使用对虾基因和白斑杆状病毒基因的稀有密码子.试验表明在该基因的前导序列中存在或不存在小顺反子时,该基因都可在大肠杆菌中诱导表达,但两者的表达量不同.小顺反子可能对基因的表达具有调控作用.
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| 关 键 词： | 对虾白斑杆状病毒 小顺反子 密码子使用频率 表达 |
| 文章编号： | 0253-4193(2001)01-0073-06 |
| 收稿时间： | 1999/6/15 0:00:00 |
| 修稿时间： | 1999年6月15日 |
The minicistron from a gene of prawn white spot bacilliform virus(WSBV) and its expression |
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| ZHANG Xiao-bo,XU Xun and YANG Feng.The minicistron from a gene of prawn white spot bacilliform virus(WSBV) and its expression[J].Acta Oceanologica Sinica (in Chinese),2001,23(1):73-78. |
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| Authors: | ZHANG Xiao-bo XU Xun and YANG Feng |
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| Institution: | 1.School of Life Science, University of Science and Technology of China, Hefei 230026, China2.School of Life Science, University of Science and Technology of China, Hefei 230026, China;Key Laboratory of Marine Biotechnology, Third Institute of Oceanography, State Oceanic Administration, Xaimen 361005, China3.Key Laboratory of Marine Biotechnology, Third Institute of Oceanography, State Oceanic Administration, Xaimen 361005, China |
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| Abstract: | There exists a minicistron in the leading sequence of p204 gene through the sequencing and analysis of libraries of DNA and cDNA from WSBV, p204 gene is predicted to be a membrane polypeptide gene. The minicistron utilizes unusual codons of genes from prawn and WSBV. The P204 genes with and without the miniciston in their leading sequence can be expressed in E. coli. But the amount of expressed products between them are different. Maybe the minicistron controls the expression of p204 gene. |
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| Keywords: | prawn whit spot bacilliform virus minicistron frequency of codon expression |
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