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光强和二氧化碳浓度变化对浒苔幼苗生长及生理的影响
引用本文:高秀秀,李亚鹤,段维军,徐年军.光强和二氧化碳浓度变化对浒苔幼苗生长及生理的影响[J].海洋学报,2015,37(10):80-87.
作者姓名:高秀秀  李亚鹤  段维军  徐年军
作者单位:1.宁波大学 应用海洋生物技术教育部重点实验室, 浙江 宁波 315211;浙江省海洋生物工程重点实验室, 浙江 宁波 315211
基金项目:国家自然科学基金项目(40876073, 41276122);国家教育部博士点基金博导基金(20123305110002); 宁波大学学科项目(XKL14D2085);宁波大学科研启动项目(F01259144702)。
摘    要:大气CO2浓度升高引起的海洋酸化如何在光变环境下影响大型海藻固碳量的问题,关系到未来海洋初级生产力的变化趋势。为研究大型海藻对CO2浓度升高和光强变化的响应,本文选取浒苔(Ulva prolifera)幼苗为实验材料,探讨其在不同光强下80、260μmol/(m2·s)]和两种CO2浓度(正常CO2浓度:400μL/L和高CO2浓度:1 000μL/L)下的生理变化。研究发现,在正常CO2浓度、高光条件下,浒苔幼苗的生长最快,超氧化物特化酶(SOD)活性最高,而过氧化氢酶(CAT)活性在低光、高CO2处理下有最大值。光合色素含量和光系统Ⅱ的光化学效率在不同处理间没有显著性差异,但叶绿素a与类胡萝卜素的比值在低光正常CO2处理下有最大值。同时,高光高CO2处理下,浒苔幼苗的可溶性蛋白含量最低。

关 键 词:CO2    光强    浒苔幼苗    叶绿素荧光效率    抗氧化活性
收稿时间:2015/2/11 0:00:00

The effects of light and increased CO2 on the growth and physiological performances in marine green algae Ulva prolifera seedling
Gao Xiuxiu,Li Yahe,Duan Weijun and Xu Nianjun.The effects of light and increased CO2 on the growth and physiological performances in marine green algae Ulva prolifera seedling[J].Acta Oceanologica Sinica (in Chinese),2015,37(10):80-87.
Authors:Gao Xiuxiu  Li Yahe  Duan Weijun and Xu Nianjun
Institution:1.Key Laboratory of Applied Marine Biotechnology of Ministry of Education, Ningbo University, Ningbo 315211, China;Key Laboratory of Marine Biotechnology of Zhejiang Province, Ningbo 315211, China2.Ningbo Entry-Exit Inspection and Quarantine Bureau Technology Centre, Ningbo 315012, China
Abstract:Ocean acidification due to increasing anthropogenic CO2 is expected to influence marine primary productivity. In order to investigate the interactive effects of increased light and CO2 changes on macroalgae,we grew Ulva prolifera seedling under the normal CO2 level (400 μL/L) and elevated CO2 level (1 000 μL/L) conditions,the light levels set as 80 and 260 μmol/(m2·s),and measured its physiological performance. Research finding that the specific growth rate and activity of SOD was highest under high light and normal CO2 level . However,the activity of CAT showed the highest value under the high CO2 and low light conditions. Under the different treatments,there was no significant effect on the chlorophyll a and maximal photochemical yield of PSⅡ (Fv/Fm),while the largest ratios of chlorophylla to carotenoid occurred at nomal CO2 and low light conditions. Meanwhile,the content of soluble protein was decreased by high light and high CO2.
Keywords:CO2  light  Ulva prolifera seedling  chlorophyll fluorescence efficiency  Antioxidant activity
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