| 羟基自由基杀灭压载水中有害赤潮物种的生物有效性研究 |
| |
| 引用本文： | 程超,白敏冬,郑琦琳,陈操,孟祥盈,张芝涛.羟基自由基杀灭压载水中有害赤潮物种的生物有效性研究[J].海洋学报,2016,38(2):131-137. |
| |
| 作者姓名： | 程超 白敏冬 郑琦琳 陈操 孟祥盈 张芝涛 |
| |
| 作者单位： | 1.大连海事大学 轮机工程学院, 辽宁 大连 116026 |
| |
| 基金项目： | 国家科技支撑计划(2013BAC06B01,2013BAC06B02);国家重大科研仪器研制项目(NSFC 61427804);国家杰出青年科学基金(NSFC 61025001);海洋科学研究公共利益的专项基金。 |
| |
| 摘 要： | 本研究采用大气压下强电离放电协同气液混溶技术,高效制备羟基自由基(·OH)杀灭3个门的典型有害赤潮物种,使用荧光染色、测定光合作用潜能等生物学检测方法确定·OH致死阈值。结果表明,5.05×104 cells/mL的赤潮异弯藻(Heterosigma akashiwo)、5.28×104 cells/mL的亚历山大藻(Alexandrium tamarense)和5.02×104 cells/mL的中肋骨条藻(Skeletonema costatum),其致死阈值分别为1.24 mg/L、2.01 mg/L、1.12 mg/L,此时其叶绿素a分解率分别为77%、85%和74%。利用光学显微镜观察,处理前后藻细胞结构有明显的改变。因此,·OH致死方法可有效地杀灭压载水中的有害赤潮藻。
|
| 关 键 词： | 羟基自由基 赤潮藻 致死阈值 |
| 收稿时间： | 6/9/2015 12:00:00 AM |
| 修稿时间： | 2015/9/23 0:00:00 |
Research on biological effectiveness of hydroxyl radicals killing marine red tide algae in ballast water |
| |
| Cheng Chao,Bai Mindong,Zheng Qilin,Chen Cao,Meng Xiangying and Zhang Zhitao.Research on biological effectiveness of hydroxyl radicals killing marine red tide algae in ballast water[J].Acta Oceanologica Sinica (in Chinese),2016,38(2):131-137. |
| |
| Authors: | Cheng Chao Bai Mindong Zheng Qilin Chen Cao Meng Xiangying and Zhang Zhitao |
| |
| Institution: | 1.College of Marine Engineering, Dalian Maritime University, Dalian 116026, China2.College of Environment and Ecology, Xiamen University, Xiamen 361005, China;Environmental Engineering Institute, Dalian Maritime University, Dalian 116026, China3.College of Environment and Ecology, Xiamen University, Xiamen 361005, China4.Environmental Engineering Institute, Dalian Maritime University, Dalian 116026, China |
| |
| Abstract: | In this study, the three typical red tide species were chosen as the experiment algae and the inactivation was achieved by hydroxyl radials generated from a strong ionization discharge combined with hydrodynamic cavitations. The viability and integrity of the algae were determined by the fluorescence staining and Pulse Amplitude Modulation. The results suggest that a quick and complete loss of viability was achieved for three species after exposure to hydroxyl radical, for the Heterosigma akashiwo, Alexandrium tamarensem, Skelrtonema costatum which the density is 5.05×104 cells/mL, 5.28×104 cells/mL, 5.02×104 cells/mL respectively, the lethal thresholds are 1.24 mg/L, 2.01mg/L, and 1.12 mg/L separately. Meanwhile Algal cells were deformed and shrunk after ·OH attack and chlorophyll content was degraded at the same time. The chlorophyll content decomposition rate reaches to 77%, 85% and 74% at the lethal thresholds. Above all, the use of hydroxyl radicals is an efficient method to kill red tide species in ballast water. |
| |
| Keywords: | hydroxyl radials red tide algae lethal thresholds |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《海洋学报》浏览原始摘要信息 |
| 点击此处可从《海洋学报》下载免费的PDF全文 |
|
